Toxoplasma evacuoles: A two-step process of secretion and fusion forms the parasitophorous vacuole

Sebastian Håkansson, Audra J. Charron, L. David Sibley

Research output: Contribution to journalArticle

210 Scopus citations

Abstract

Rapid discharge of secretory organelles called rhoptries is tightly coupled with host cell entry by the protozoan parasite Toxoplasma gondii. Rhoptry contents were deposited in clusters of vesicles within the host cell cytosol and within the parasitophorous vacuole. To examine the fate of these rhoptry-derived secretory vesicles, we utilized cytochalasin D to prevent invasion, leading to accumulation of protein-rich vesicles in the host cell cytosol. These vesicles lack an internal parasite and are hence termed evacuoles. Like the mature parasite-containing vacuole, evacuoles became intimately associated with host cell mitochondria and endoplasmic reticulum, while remaining completely resistant to fusion with host cell endosomes and lysosomes. In contrast, evacuoles were recruited to pre-existing, parasite-containing vacuoles and were capable of fusing and delivering their contents to these compartments. Our findings indicate that a two-step process involving direct rhoptry secretion into the host cell cytoplasm followed by incorporation into the vacuole generates the parasitophorous vacuole occupied by Toxoplasma. The characteristic properties of the mature vacuole are likely to be determined by this early delivery of rhoptry components.

Original languageEnglish
Pages (from-to)3132-3144
Number of pages13
JournalEMBO Journal
Volume20
Issue number12
DOIs
StatePublished - Jun 15 2001

Keywords

  • Exocytosis
  • Mitochondria
  • Parasitophorous vacuole
  • Rhoptry
  • Trafficking

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