TY - JOUR
T1 - Toxin Genotypes and Plasmid Profiles As Determinants of Systemic Sequelae in Escherichia Coli 0157:H7 Infections
AU - Ostroff, Stephen M.
AU - Tarr, Phillip I.
AU - Neill, Marguerite A.
AU - Lewis, Jay H.
AU - Hargrett-Bean, Nancy
AU - Kobayashi, John M.
N1 - Funding Information:
Received for publication 6 February 1989 and in revised form 26 June 1989. This work was supported in part by grant RR-05655 from the Division of Research Resources, National Institutes of Health. Please address requests for reprints to Dr. Stephen M. Ostroff, Centers for Disease Control, 1600 Clifton Road NE, Building 1/5124, Mailstop C08, Atlanta, GA 30333.
PY - 1989/12
Y1 - 1989/12
N2 - In 1987, 93 Escherichia coli 0157:H7 isolates were collected during routine surveillance for this pathogen in the state of Washington. Toxin genotypes and plasmid profiles were correlated with the clinical sequelae of illness in 88 of the 93 patients from whom these strains were isolated. Thirteen plasmid patterns were observed among the 88 tested isolates; four patterns accounted for 82*% of the isolates. Genetic probing for Shiga-like toxins (SLT) I and II demonstrated the presence of both genes in 67 (76%), SLTI alone in three (3%), and SLT II alone in 18 (20%). The hemolytic uremic syndrome or thrombotic thrombocytopenic purpura developed in seven (39%) of 18 patients infected with isolates having only the SLT II gene, while these complications occurred in only four (6%) of 70 patients infected with isolates having the other two genotypes (relative risk, 6.8; 95% confidence interval, 1.9, 26.4). This study shows that E. coli 0157:H7 isolates systematically collected from a single geographic region over a defined time period exhibit considerable diversity in plasmid content and toxin genotype and that the toxin genotype of the infecting strain may influence the risk of developing microangiopathic sequelae.
AB - In 1987, 93 Escherichia coli 0157:H7 isolates were collected during routine surveillance for this pathogen in the state of Washington. Toxin genotypes and plasmid profiles were correlated with the clinical sequelae of illness in 88 of the 93 patients from whom these strains were isolated. Thirteen plasmid patterns were observed among the 88 tested isolates; four patterns accounted for 82*% of the isolates. Genetic probing for Shiga-like toxins (SLT) I and II demonstrated the presence of both genes in 67 (76%), SLTI alone in three (3%), and SLT II alone in 18 (20%). The hemolytic uremic syndrome or thrombotic thrombocytopenic purpura developed in seven (39%) of 18 patients infected with isolates having only the SLT II gene, while these complications occurred in only four (6%) of 70 patients infected with isolates having the other two genotypes (relative risk, 6.8; 95% confidence interval, 1.9, 26.4). This study shows that E. coli 0157:H7 isolates systematically collected from a single geographic region over a defined time period exhibit considerable diversity in plasmid content and toxin genotype and that the toxin genotype of the infecting strain may influence the risk of developing microangiopathic sequelae.
UR - http://www.scopus.com/inward/record.url?scp=0024369158&partnerID=8YFLogxK
U2 - 10.1093/infdis/160.6.994
DO - 10.1093/infdis/160.6.994
M3 - Article
C2 - 2685131
AN - SCOPUS:0024369158
VL - 160
SP - 994
EP - 998
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
SN - 0022-1899
IS - 6
ER -