Toward invivo detection of hydrogen peroxide with ultrasound molecular imaging

Emilia S. Olson, Jahir Orozco, Zhe Wu, Christopher D. Malone, Boemha Yi, Wei Gao, Mohammad Eghtedari, Joseph Wang, Robert F. Mattrey

Research output: Contribution to journalArticlepeer-review

89 Scopus citations


We present a new class of ultrasound molecular imaging agents that extend upon the design of micromotors that are designed to move through fluids by catalyzing hydrogen peroxide (H2O2) and propelling forward by escaping oxygen microbubbles. Micromotor converters require 62mm of H2O2 to move - 1000-fold higher than is expected invivo. Here, we aim to prove that ultrasound can detect the expelled microbubbles, to determine the minimum H2O2 concentration needed for microbubble detection, explore alternate designs to detect the H2O2 produced by activated neutrophils and perform preliminary invivo testing. Oxygen microbubbles were detected by ultrasound at 2.5mm H2O2. Best results were achieved with a 400-500nm spherical design with alternating surface coatings of catalase and PSS over a silica core. The lowest detection limit of 10-100μm was achieved when assays were done in plasma. Using this design, we detected the H2O2 produced by freshly isolated PMA-activated neutrophils allowing their distinction from naïve neutrophils. Finally, we were also able to show that direct injection of these nanospheres into an abscess invivo enhanced ultrasound signal only when they contained catalase, and only when injected into an abscess, likely because of the elevated levels of H2O2 produced by inflammatory mediators.

Original languageEnglish
Pages (from-to)8918-8924
Number of pages7
Issue number35
StatePublished - Nov 2013


  • Abscess
  • Hydrogen peroxide
  • Molecular imaging
  • Nanotechnology
  • Ultrasound


Dive into the research topics of 'Toward invivo detection of hydrogen peroxide with ultrasound molecular imaging'. Together they form a unique fingerprint.

Cite this