Toward gene therapy of endometriosis: transductional and transcriptional targeting of adenoviral vectors to endometriosis cells

Essam Eldin R. Othman, Zeng B. Zhu, David T. Curiel, Nilufar Khatoon, Hosam T. Salem, Essam Al Din M. Khalifa, Ayman Al-Hendy

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Objective: The purpose of this study was to screen a panel of targeted adenoviruses as vectors for endometriosis gene therapy. Study Design: Endometriotic cells were obtained from subjects with ovarian endometriomas. Liver tissues were taken from donors during hepatic transplantation surgery. Human endometriotic cells and liver tissues were transfected by targeted adenoviruses expressing luciferase reporter gene. Luciferase activity that was mediated by each virus was expressed as a percentage of adenovirus serotype 5 (Ad5-CMV-luc) activity. The 2-tailed Studentt test was used to compare the adenovirus data. Results: In endometriotic cells, the adenovirus-RGD (Ad-RGD-luc), adenovirus under secretory leukocyte protease inhibitor promoter (Ad-SLPI-luc), and adenovirus under heparanase promoter (Ad-heparanase-luc) showed significantly higher activity, compared with the adenovirus serotype 5. In liver tissues, adenovirus-survivin (Ad-survivin-luc) and Ad-heparanase-luc had significantly lower activity, compared with adenovirus serotype 5. Conclusion: Ad-heparanase-luc showed "endometriosis on, liver off" phenotype and is a promising vector for endometriosis gene therapy.

Original languageEnglish
Pages (from-to)117.e1-117.e6
JournalAmerican journal of obstetrics and gynecology
Volume199
Issue number2
DOIs
StatePublished - Aug 2008

Keywords

  • adenovirus
  • endometriosis
  • gene therapy
  • targeting strategy

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