TY - JOUR
T1 - Topical formulation containing naringenin
T2 - Efficacy against ultraviolet B irradiation-induced skin inflammation and oxidative stress in mice
AU - Martinez, Renata M.
AU - Pinho-Ribeiro, Felipe A.
AU - Steffen, Vinicius S.
AU - Silva, Thais C.C.
AU - Caviglione, Carla V.
AU - Bottura, Carolina
AU - Fonseca, Maria J.V.
AU - Vicentini, Fabiana T.M.C.
AU - Vignoli, Josiane A.
AU - Baracat, Marcela M.
AU - Georgetti, Sandra R.
AU - Verri, Waldiceu A.
AU - Casagrande, Rubia
N1 - Funding Information:
This work was supported by Brazilian grants from Coordenadoria de Aperfei?oamento de Pessoal de N?vel Superior (CAPES), Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq), Ministe?rio da Cie?ncia, Tecnologia e Inova??o (MCTI)/Secretaria da Cie?ncia, Tecnologia e Inova??o (SETI)/Funda??o Arauca?ria, and Parana? State Government. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Farmacore Biotecnologia LTDA provided support in the form of salaries for authors [FTMCV], but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of these authors are articulated in the ''author contributions'' section. The authors have the following interests: Fabiana T. M. C. Vicentini is employed by Farmacore Biotecnologia LTDA. There are no patents, products in development or marketed products to declare. This does not alter the authors'' adherence to all the PLOS ONE policies on sharing data and materials, as detailed online in the guide for authors. We thank Marcelo Tempesta Oliveira, Denise Duarte, and Cristina Aparecida Lopes for their technical support to this research.
Publisher Copyright:
© 2016 Martinez et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2016/1/7
Y1 - 2016/1/7
N2 - Naringenin (NGN) exhibits anti-inflammatory and antioxidant activities, but it remains undetermined its topical actions against ultraviolet B (UVB)-induced inflammation and oxidative stress in vivo. The purpose of this study was to evaluate the physicochemical and functional antioxidant stability of NGN containing formulations, and the effects of selected NGN containing formulation on UVB irradiation-induced skin inflammation and oxidative damage in hairless mice. NGN presented ferric reducing power, ability to scavenge 2,20-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and hydroxyl radical, and inhibited iron-independent and dependent lipid peroxidation. Among the three formulations containing NGN, only the F3 kept its physicochemical and functional stability over 180 days. Topical application of F3 in mice protected from UVB-induced skin damage by inhibiting edema and cytokine production (TNF-α, IL-1β, IL-6, and IL-10). Furthermore, F3 inhibited superoxide anion and lipid hydroperoxides production and maintained ferric reducing and ABTS scavenging abilities, catalase activity, and reduced glutathione levels. In addition, F3 maintained mRNA expression of cellular antioxidants glutathione peroxidase 1, glutathione reductase and transcription factor Nrf2 (nuclear factor erythroid 2-related factor 2), and induced mRNA expression of heme oxygenase-1. In conclusion, a formulation containing NGN may be a promising approach to protecting the skin from the deleterious effects of UVB irradiation.
AB - Naringenin (NGN) exhibits anti-inflammatory and antioxidant activities, but it remains undetermined its topical actions against ultraviolet B (UVB)-induced inflammation and oxidative stress in vivo. The purpose of this study was to evaluate the physicochemical and functional antioxidant stability of NGN containing formulations, and the effects of selected NGN containing formulation on UVB irradiation-induced skin inflammation and oxidative damage in hairless mice. NGN presented ferric reducing power, ability to scavenge 2,20-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and hydroxyl radical, and inhibited iron-independent and dependent lipid peroxidation. Among the three formulations containing NGN, only the F3 kept its physicochemical and functional stability over 180 days. Topical application of F3 in mice protected from UVB-induced skin damage by inhibiting edema and cytokine production (TNF-α, IL-1β, IL-6, and IL-10). Furthermore, F3 inhibited superoxide anion and lipid hydroperoxides production and maintained ferric reducing and ABTS scavenging abilities, catalase activity, and reduced glutathione levels. In addition, F3 maintained mRNA expression of cellular antioxidants glutathione peroxidase 1, glutathione reductase and transcription factor Nrf2 (nuclear factor erythroid 2-related factor 2), and induced mRNA expression of heme oxygenase-1. In conclusion, a formulation containing NGN may be a promising approach to protecting the skin from the deleterious effects of UVB irradiation.
UR - http://www.scopus.com/inward/record.url?scp=84954179895&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0146296
DO - 10.1371/journal.pone.0146296
M3 - Article
C2 - 26741806
AN - SCOPUS:84954179895
SN - 1932-6203
VL - 11
JO - PloS one
JF - PloS one
IS - 1
M1 - e0146296
ER -