TY - JOUR
T1 - Toll-like Receptor-Mediated Responses by Placental Hofbauer Cells (HBCs)
T2 - A Potential Pro-Inflammatory Role for Fetal M2 Macrophages
AU - Young, Omar M.
AU - Tang, Zhonghua
AU - Niven-Fairchild, Tracy
AU - Tadesse, Serkalem
AU - Krikun, Graciela
AU - Norwitz, Errol R.
AU - Mor, Gil
AU - Abrahams, Vikki M.
AU - Guller, Seth
N1 - Publisher Copyright:
© 2014 The Authors.
PY - 2015/1/1
Y1 - 2015/1/1
N2 - Problem: Microbial-driven responses in placenta are linked with adverse pregnancy outcomes. The role of Toll-like receptor (TLR) function in Hofbauer cells (HBCs) and fetal macrophages of the placental villous core remains understudied. Method of Study: Flow cytometry and immunohistochemistry (IHC) were used to establish the phenotype of HBCs. Regulation of cytokine secretion in response to treatment with TLR agonists and expression levels of TLRs and co-activators were compared in HBCs, placental fibroblasts (FIBs), and human umbilical vein endothelial cells (HUVECs) using ELISA and qPCR. Results: Although flow cytometry and IHC revealed HBCs to be M2 (anti-inflammatory) macrophages, LPS and polyinosinic: polycytidylic acid [poly (I:C)] treatments markedly enhanced IL-6 secretion by HBCs, and expression of TLR-2, TLR-3, TLR-4, CD14, and MD-2 was the highest in HBCs. Conclusion: These results indicate that although HBCs are M2 macrophages, inflammatory responses are induced through TLR-3 and TLR-4 in this cell type, suggesting a role in microbial-driven placental/fetal inflammation.
AB - Problem: Microbial-driven responses in placenta are linked with adverse pregnancy outcomes. The role of Toll-like receptor (TLR) function in Hofbauer cells (HBCs) and fetal macrophages of the placental villous core remains understudied. Method of Study: Flow cytometry and immunohistochemistry (IHC) were used to establish the phenotype of HBCs. Regulation of cytokine secretion in response to treatment with TLR agonists and expression levels of TLRs and co-activators were compared in HBCs, placental fibroblasts (FIBs), and human umbilical vein endothelial cells (HUVECs) using ELISA and qPCR. Results: Although flow cytometry and IHC revealed HBCs to be M2 (anti-inflammatory) macrophages, LPS and polyinosinic: polycytidylic acid [poly (I:C)] treatments markedly enhanced IL-6 secretion by HBCs, and expression of TLR-2, TLR-3, TLR-4, CD14, and MD-2 was the highest in HBCs. Conclusion: These results indicate that although HBCs are M2 macrophages, inflammatory responses are induced through TLR-3 and TLR-4 in this cell type, suggesting a role in microbial-driven placental/fetal inflammation.
KW - Hofbauer cells
KW - Macrophages
KW - Placenta
KW - TLRs
UR - http://www.scopus.com/inward/record.url?scp=84928349520&partnerID=8YFLogxK
U2 - 10.1111/aji.12336
DO - 10.1111/aji.12336
M3 - Article
C2 - 25345551
AN - SCOPUS:84928349520
SN - 1046-7408
VL - 73
SP - 22
EP - 35
JO - American Journal of Reproductive Immunology
JF - American Journal of Reproductive Immunology
IS - 1
ER -