TY - JOUR
T1 - Tissue-specific smooth muscle cell subtypes identified by transcriptional profiling
AU - Lin, Chien Jung
AU - Mecham, Robert P.
N1 - Funding Information:
This study was funded by National Institutes of Health grants R01HL53325 and funds from the Ines Mandl Research Foundation (RPM) . C-JL was supported by T32HL007081 and T32HL125241 . The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
Publisher Copyright:
© 2021 Elsevier Ltd
PY - 2021/10
Y1 - 2021/10
N2 - Smooth muscle cells (SMCs) are specialized cells present in many organs where they serve diverse tissue-specific functions. Using the Tabula Muris compendium of single-cell RNA sequencing data, we extracted individual SMC transcriptomes from eight mouse tissues to investigate the transcriptomic landscape of tissue-specific SMCs. We identified marker genes, signaling pathways, and biological processes enriched in tissue-specific SMCs, and inferred potential ligand-receptor interaction between SMC and other cell types. Our analysis also identified sex differences in SMC gene expression in different tissues. Lastly, we used unsupervised clustering to identify novel SMC subtypes based on their downstream targets of transcription factors. Our results highlight the variable SMC phenotypes and underscore this cell's remarkable adaptability to contribute to diverse tissue function.
AB - Smooth muscle cells (SMCs) are specialized cells present in many organs where they serve diverse tissue-specific functions. Using the Tabula Muris compendium of single-cell RNA sequencing data, we extracted individual SMC transcriptomes from eight mouse tissues to investigate the transcriptomic landscape of tissue-specific SMCs. We identified marker genes, signaling pathways, and biological processes enriched in tissue-specific SMCs, and inferred potential ligand-receptor interaction between SMC and other cell types. Our analysis also identified sex differences in SMC gene expression in different tissues. Lastly, we used unsupervised clustering to identify novel SMC subtypes based on their downstream targets of transcription factors. Our results highlight the variable SMC phenotypes and underscore this cell's remarkable adaptability to contribute to diverse tissue function.
KW - Extracellular matrix
KW - Single cell RNA sequencing
KW - Smooth muscle cell
KW - Tabula Muris project
KW - Transcriptomics
UR - http://www.scopus.com/inward/record.url?scp=85113192705&partnerID=8YFLogxK
U2 - 10.1016/j.biocel.2021.106055
DO - 10.1016/j.biocel.2021.106055
M3 - Article
C2 - 34411694
AN - SCOPUS:85113192705
SN - 1357-2725
VL - 139
JO - International Journal of Biochemistry and Cell Biology
JF - International Journal of Biochemistry and Cell Biology
M1 - 106055
ER -