Tissue-specific expression and post-translational modification of histone H3 variants

Benjamin A. Garcia, C. Eric Thomas, Neil L. Kelleher, Craig A. Mizzen

Research output: Contribution to journalArticlepeer-review

57 Scopus citations


Analyses of histone H3 from 10 rat tissues using a Middle Down proteomics platform revealed tissue-specific differences in their expression and global PTM abundance. ESI/FTMS with electron capture dissociation showed that, in general, these proteins were hypomodified in heart, liver and testes. H3.3 was hypermodified compared to H3.2 in some, but not all tissues. In addition, a novel rattestes-specific H3 protein was identified with this approach.

Original languageEnglish
Pages (from-to)4225-4236
Number of pages12
JournalJournal of Proteome Research
Issue number10
StatePublished - Oct 2008


  • Electron capture dissociation
  • Histone
  • Mass spectrometry
  • Post-translational modification
  • Proteomic
  • Variants


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