TY - JOUR
T1 - Time-restricted feeding without reducing caloric intake prevents metabolic diseases in mice fed a high-fat diet
AU - Hatori, Megumi
AU - Vollmers, Christopher
AU - Zarrinpar, Amir
AU - DiTacchio, Luciano
AU - Bushong, Eric A.
AU - Gill, Shubhroz
AU - Leblanc, Mathias
AU - Chaix, Amandine
AU - Joens, Matthew
AU - Fitzpatrick, James A.J.
AU - Ellisman, Mark H.
AU - Panda, Satchidananda
N1 - Funding Information:
We thank Drs. Marc Montminy and Ron Evans for helpful comments and advice on the work and for sharing their research equipment. We also thank Hiep Le, Sheena Keding, Chrissta Maracle, and Ishika Arora for technical support. The IMOD stereology plug-in was developed by Andrew Noske, and we thank him for providing instruction and guidance. This work was partially supported by the Pew Scholars Program in Biomedical Sciences, NIH grant DK091618, Sanofi Discovery Innovation Grant, and The Anderson Foundation support to S.P.; The Japan Society for the Promotion of Science (JSPS) Fellowship to M.H.; The Blasker-Rose-Miah Fund of The San Diego Foundation to C.V.; NIH T32 DK07202 training grant to A.Z.; and NCRR grant 5P41RR004050 to M.H.E.
PY - 2012/6/6
Y1 - 2012/6/6
N2 - While diet-induced obesity has been exclusively attributed to increased caloric intake from fat, animals fed a high-fat diet (HFD) ad libitum (ad lib) eat frequently throughout day and night, disrupting the normal feeding cycle. To test whether obesity and metabolic diseases result from HFD or disruption of metabolic cycles, we subjected mice to either ad lib or time-restricted feeding (tRF) of a HFD for 8 hr per day. Mice under tRF consume equivalent calories from HFD as those with ad lib access yet are protected against obesity, hyperinsulinemia, hepatic steatosis, and inflammation and have improved motor coordination. The tRF regimen improved CREB, mTOR, and AMPK pathway function and oscillations of the circadian clock and their target genes' expression. These changes in catabolic and anabolic pathways altered liver metabolome and improved nutrient utilization and energy expenditure. We demonstrate in mice that tRF regimen is a nonpharmacological strategy against obesity and associated diseases.
AB - While diet-induced obesity has been exclusively attributed to increased caloric intake from fat, animals fed a high-fat diet (HFD) ad libitum (ad lib) eat frequently throughout day and night, disrupting the normal feeding cycle. To test whether obesity and metabolic diseases result from HFD or disruption of metabolic cycles, we subjected mice to either ad lib or time-restricted feeding (tRF) of a HFD for 8 hr per day. Mice under tRF consume equivalent calories from HFD as those with ad lib access yet are protected against obesity, hyperinsulinemia, hepatic steatosis, and inflammation and have improved motor coordination. The tRF regimen improved CREB, mTOR, and AMPK pathway function and oscillations of the circadian clock and their target genes' expression. These changes in catabolic and anabolic pathways altered liver metabolome and improved nutrient utilization and energy expenditure. We demonstrate in mice that tRF regimen is a nonpharmacological strategy against obesity and associated diseases.
UR - https://www.scopus.com/pages/publications/84862008430
U2 - 10.1016/j.cmet.2012.04.019
DO - 10.1016/j.cmet.2012.04.019
M3 - Article
C2 - 22608008
AN - SCOPUS:84862008430
SN - 1550-4131
VL - 15
SP - 848
EP - 860
JO - Cell metabolism
JF - Cell metabolism
IS - 6
ER -