Time-resolved spectroscopy and near infrared imaging enhanced by receptor-targeted contrast agents for prostate cancer detection

Y. Pu, W. B. Wang, G. C. Tang, S. Achilefu, R. R. Alfano

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

8 Scopus citations

Abstract

Time-resolved spectroscopy and near infrared imaging enhanced by receptor-targeted contrast agents for prostate cancer detection will be presented. Two contrast agents, Cybesin and Cytate, were investigated using time-resolved spectroscopy in aqueous solution and cancerous and normal prostate tissues. The time evolution of the fluorescent dipole in solution was studied using a system of first-order linear differential equations containing two main parameters: the decay rate of emission and the rate of one orthogonal emission component transferring to another. An analytical polarization model was developed and used to extract rotational times and fluorescence anisotropies of the contrast agents in prostate tissues. The differences of rotational times and polarization anisotropies were observed for Cybesin (Cytate) in cancerous and normal prostate tissue, which reflect preferred bond of contrast agents and cancerous tissue cells. The conjugation of Cybesin (Cytate) to prostate cancerous cells offers high contrast between normal and cancerous tissues.

Original languageEnglish
Title of host publicationOptical Biopsy IX
DOIs
StatePublished - Apr 21 2011
EventOptical Biopsy IX - San Francisco, CA, United States
Duration: Jan 24 2011Jan 26 2011

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7895
ISSN (Print)1605-7422

Conference

ConferenceOptical Biopsy IX
Country/TerritoryUnited States
CitySan Francisco, CA
Period01/24/1101/26/11

Keywords

  • polarization anisotropy
  • prostate cancer receptor-targeted contrast agents
  • rotation time
  • time-resolved fluorescence polarization kinetics

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