@inbook{3cbb881605094e5abd18adc93992c18e,
title = "Time-resolved cryo-electron microscopy using a microfluidic chip",
abstract = "With the advent of direct electron detectors, cryo-EM has become a popular choice for molecular structure determination. Among its advantages over X-ray crystallography are (1) no need for crystals, (2) much smaller sample volumes, and (3) the ability to determine multiple structures or conformations coexisting in one sample. In principle, kinetic experiments can be done using standard cryo-EM, but mixing and freezing grids require several seconds. However, many biological processes are much faster than that time scale, and the ensuing short-lived states of the molecules cannot be captured. Here, we lay out a detailed protocol for how to capture such intermediate states on the millisecond time scale with time-resolved cryo-EM.",
keywords = "Microfluidic chip, Short-lived intermediates heterogeneity, Single-particle cryo-EM, Spraying, Time-resolved",
author = "Sandip Kaledhonkar and Ziao Fu and Howard White and Joachim Frank",
note = "Publisher Copyright: {\textcopyright} 2018, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2018",
doi = "10.1007/978-1-4939-7759-8_4",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "59--71",
booktitle = "Methods in Molecular Biology",
}