TY - JOUR
T1 - Time-dependent blood flow and oxygenation in human skeletal muscles measured with noninvasive near-infrared diffuse optical spectroscopies
AU - Yu, Guoqiang
AU - Durduran, Turgut
AU - Lech, Gwen
AU - Zhou, Chao
AU - Chance, Britton
AU - Mohler, Emile R.
AU - Yodh, Arjun G.
PY - 2005/3
Y1 - 2005/3
N2 - We have employed near-infrared optical methods to measure noninvasively the dynamics of muscle blood flow and oxygen saturation (StO 2) during cuff occlusion and plantar flexion exercise. Relative muscle oxygen consumption (rVO2) was also computed from these data. Diffuse correlation spectroscopy provides information about blood flow, and diffuse reflectance spectroscopy provides information about blood oxygenation. Ten healthy subjects and one patient with peripheral arterial disease (PAD) were studied during 3-min arterial cuff occlusion of arm and leg, and during 1-min plantar flexion exercise. Signals from different layers (cutaneous tissues and muscles) during cuff occlusion were differentiated, revealing strong hemodynamic responses from muscle layers. During exercise in healthy legs, the observed ∼4.7 fold increase in relative blood flow (rBF) was significantly lower than the corresponding increase in rVO2(∼7 fold). The magnitudes of rBF and rVO2during exercise in the PAD patient were ∼1/2 of the healthy controls, and the StO2recovery time was twice that of the controls. The hybrid instrument improves upon current technologies for measuring muscle responses by simultaneously measuring rBF and S tO2. The instrument thus provides a method for evaluation of microcirculation and muscle metabolism in patients with vascular diseases.
AB - We have employed near-infrared optical methods to measure noninvasively the dynamics of muscle blood flow and oxygen saturation (StO 2) during cuff occlusion and plantar flexion exercise. Relative muscle oxygen consumption (rVO2) was also computed from these data. Diffuse correlation spectroscopy provides information about blood flow, and diffuse reflectance spectroscopy provides information about blood oxygenation. Ten healthy subjects and one patient with peripheral arterial disease (PAD) were studied during 3-min arterial cuff occlusion of arm and leg, and during 1-min plantar flexion exercise. Signals from different layers (cutaneous tissues and muscles) during cuff occlusion were differentiated, revealing strong hemodynamic responses from muscle layers. During exercise in healthy legs, the observed ∼4.7 fold increase in relative blood flow (rBF) was significantly lower than the corresponding increase in rVO2(∼7 fold). The magnitudes of rBF and rVO2during exercise in the PAD patient were ∼1/2 of the healthy controls, and the StO2recovery time was twice that of the controls. The hybrid instrument improves upon current technologies for measuring muscle responses by simultaneously measuring rBF and S tO2. The instrument thus provides a method for evaluation of microcirculation and muscle metabolism in patients with vascular diseases.
KW - Arterial cuff occlusion
KW - Diffuse correlation spectroscopy
KW - Diffuse reflectance spectroscopy
KW - Dynamic light scattering
KW - Light diffusion
KW - Near-infrared spectroscopy
KW - Peripheral arterial disease
KW - Photon migration
KW - Plantar flexion exercise
UR - http://www.scopus.com/inward/record.url?scp=24744439989&partnerID=8YFLogxK
U2 - 10.1117/1.1884603
DO - 10.1117/1.1884603
M3 - Article
C2 - 15910100
AN - SCOPUS:24744439989
SN - 1083-3668
VL - 10
JO - Journal of biomedical optics
JF - Journal of biomedical optics
IS - 2
M1 - 024027
ER -