Three-dimensional super-resolution imaging with a corkscrew point spread function

Matthew D. Lew; Steven F. Lee; W. E. Moerner

doi:10.1364/ntm.2011.nmb2

Three-dimensional super-resolution imaging with a corkscrew point spread function

Matthew D. Lew, Steven F. Lee, W. E. Moerner

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

We describe the design of a corkscrew point spread function for 3D super-resolution microscopy. To prove the principle, we image fluorescent beads on a patterned PDMS surface, achieving a localization precision of 3 nm in x, 2 nm in y, and 6 nm in z.

Original language	English
Title of host publication	Novel Techniques in Microscopy, NTM 2011
Publisher	Optical Society of America (OSA)
ISBN (Print)	9781557529091
DOIs	https://doi.org/10.1364/ntm.2011.nmb2
State	Published - 2011
Event	Novel Techniques in Microscopy, NTM 2011 - Monterey, CA, United States Duration: Apr 4 2011 → Apr 6 2011

Publication series

Name	Optics InfoBase Conference Papers
ISSN (Electronic)	2162-2701

Conference

Conference	Novel Techniques in Microscopy, NTM 2011
Country/Territory	United States
City	Monterey, CA
Period	04/4/11 → 04/6/11

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10.1364/ntm.2011.nmb2

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@inproceedings{49c8c619afb24dcb92ef427db6b4cf93,

title = "Three-dimensional super-resolution imaging with a corkscrew point spread function",

abstract = "We describe the design of a corkscrew point spread function for 3D super-resolution microscopy. To prove the principle, we image fluorescent beads on a patterned PDMS surface, achieving a localization precision of 3 nm in x, 2 nm in y, and 6 nm in z.",

author = "Lew, {Matthew D.} and Lee, {Steven F.} and Moerner, {W. E.}",

year = "2011",

doi = "10.1364/ntm.2011.nmb2",

language = "English",

isbn = "9781557529091",

series = "Optics InfoBase Conference Papers",

publisher = "Optical Society of America (OSA)",

booktitle = "Novel Techniques in Microscopy, NTM 2011",

note = "null ; Conference date: 04-04-2011 Through 06-04-2011",

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AU - Lew, Matthew D.

AU - Lee, Steven F.

AU - Moerner, W. E.

PY - 2011

Y1 - 2011

N2 - We describe the design of a corkscrew point spread function for 3D super-resolution microscopy. To prove the principle, we image fluorescent beads on a patterned PDMS surface, achieving a localization precision of 3 nm in x, 2 nm in y, and 6 nm in z.

AB - We describe the design of a corkscrew point spread function for 3D super-resolution microscopy. To prove the principle, we image fluorescent beads on a patterned PDMS surface, achieving a localization precision of 3 nm in x, 2 nm in y, and 6 nm in z.

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U2 - 10.1364/ntm.2011.nmb2

DO - 10.1364/ntm.2011.nmb2

M3 - Conference contribution

AN - SCOPUS:85087594871

SN - 9781557529091

T3 - Optics InfoBase Conference Papers

BT - Novel Techniques in Microscopy, NTM 2011

PB - Optical Society of America (OSA)

Y2 - 4 April 2011 through 6 April 2011

ER -

Three-dimensional super-resolution imaging with a corkscrew point spread function

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