Three-dimensional structure of Phyllomedusin, a NK1 receptor agonist bound to dodecylphosphocholine micelles

Anjali D. Ganjiwale, Sudha M. Cowsik

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Phyllomedusin, an amphibian tachykinin decapeptide, has been shown to be selective for Neurokinin 1 receptor. Because the micelle-associated structure may be relevant to the Phyllomedusin-receptor interaction, the three-dimensional structure of the Phyllomedusin in aqueous and micellar environments has been studied by two-dimensional proton nuclear magnetic resonance (2D 1H NMR spectroscopy) and distance geometry calculations. Sequence specific resonance assignments of protons have been made from correlation spectroscopy (TOCSY, DQF-COSY) and NOESY spectroscopy. The interproton distance constraints and dihedral angle constraints have been utilized to generate a family of structures using DYANA. The CD and NMR results show that, while in water Phyllomedusin prefers to be in an extended chain conformation, whereas in the presence of dodecylphosphocholine micelles, a membrane model system, a partial helical conformation is induced. Analysis of NMR data indicates that the global fold of Phyllomedusin can be explained in terms of equilibrium between 310-helix and α-helix from residue 4 to 10. An extended highly flexible N-terminus displays some degree of order and a possible turn structure. A comparison between the conformational features of Phyllomedusin and different Neurokinin 1 receptor agonist indicates several common features in the distribution of hydrophobic and hydrophilic residues. The conformational similarities suggest that the molecules interact with receptor in an analogous manner.

Original languageEnglish
Pages (from-to)176-184
Number of pages9
JournalJournal of Structural Biology
Volume167
Issue number2
DOIs
StatePublished - Aug 2009
Externally publishedYes

Keywords

  • 3D structure
  • Bioactive peptide
  • Circular dichroism
  • NMR spectroscopy
  • Neurokinin receptors
  • Tachykinin

Fingerprint

Dive into the research topics of 'Three-dimensional structure of Phyllomedusin, a NK1 receptor agonist bound to dodecylphosphocholine micelles'. Together they form a unique fingerprint.

Cite this