Three dimensional structure of mammalian tachykinin peptide neurokinin b bound to lipid micelles

Anil K. Mantha, Indu R. Chandrashekar, Najma Z. Baquer, Sudha M. Cowsik

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Neurokinin B (NKB), a decapeptide of mammalian origin exhibits a variety of biological activities such as regulatory functions in reproduction, pre-eclampsia and neuroprotection in Alzheimer's disease. In order to gain insight into structure-function relationship, three-dimensional structure of NKB has been investigated using CD spectropolarimetry and two-dimensional proton nuclear magnetic resonance (2D 1H-NMR) spectroscopy in aqueous and membrane mimetic solvents. Unambiguous NMR assignments of resonances have been made with the aid of correlation spectroscopy (DQF-COSY and TOCSY) experiments and Nuclear Overhauser Effect Spectroscopy (NOESY) experiments. Distance constraints obtained from the NMR data have been used to generate a family of structures, which have been refined using restrained energy minimization and dynamics. Our data show that a helical structure is induced in NKB, in presence of perdeuterated dodecyl phosphocholine (DPC) micelles, a membrane model system. Further, the conformation adopted by NKB in presence of DPC micelles represents a structural motif typical of neurokinin-3 selective agonists.

Original languageEnglish
Pages (from-to)137-147
Number of pages11
JournalJournal of Biomolecular Structure and Dynamics
Volume22
Issue number2
DOIs
StatePublished - Oct 2004
Externally publishedYes

Keywords

  • 2D-NMR spectroscopy
  • Bioactive peptides
  • Circular dichroism (CD)
  • Lipid induced structure
  • NK-3 agonist
  • Neurokinin B
  • Tachykinins

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