Three-dimensional culture systems to induce chondrogenesis of adipose-derived stem cells.

Bradley T. Estes; Farshid Guilak

doi:10.1007/978-1-61737-960-4_15

Three-dimensional culture systems to induce chondrogenesis of adipose-derived stem cells.

Bradley T. Estes
, Farshid Guilak

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

Stem cells can easily be harvested from adipose tissue in large numbers for use in tissue-engineering approaches for cartilage repair or regeneration. In this chapter, we describe in vitro tissue-engineering models that we have used in our laboratory for the chondrogenic induction of adipose-derived stem cells (ASC). In addition to the proper growth factor environment, chondrogenesis requires cells to be maintained in a rounded morphology in three-dimensional (3D) culture, and thus properties of the biomaterial scaffold also play a critical role in ASC differentiation. Histologic and immunohistologic methods for assessing chondrogenesis are also presented. In general, 10-12 weeks are required to assess ASC chondrogenesis in these model systems.

Original language	English
Pages (from-to)	201-217
Number of pages	17
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	702
DOIs	https://doi.org/10.1007/978-1-61737-960-4_15
State	Published - 2011

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abstract = "Stem cells can easily be harvested from adipose tissue in large numbers for use in tissue-engineering approaches for cartilage repair or regeneration. In this chapter, we describe in vitro tissue-engineering models that we have used in our laboratory for the chondrogenic induction of adipose-derived stem cells (ASC). In addition to the proper growth factor environment, chondrogenesis requires cells to be maintained in a rounded morphology in three-dimensional (3D) culture, and thus properties of the biomaterial scaffold also play a critical role in ASC differentiation. Histologic and immunohistologic methods for assessing chondrogenesis are also presented. In general, 10-12 weeks are required to assess ASC chondrogenesis in these model systems.",

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Three-dimensional culture systems to induce chondrogenesis of adipose-derived stem cells.

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