TY - JOUR
T1 - Three-dimensional culture systems to induce chondrogenesis of adipose-derived stem cells.
AU - Estes, Bradley T.
AU - Guilak, Farshid
PY - 2011
Y1 - 2011
N2 - Stem cells can easily be harvested from adipose tissue in large numbers for use in tissue-engineering approaches for cartilage repair or regeneration. In this chapter, we describe in vitro tissue-engineering models that we have used in our laboratory for the chondrogenic induction of adipose-derived stem cells (ASC). In addition to the proper growth factor environment, chondrogenesis requires cells to be maintained in a rounded morphology in three-dimensional (3D) culture, and thus properties of the biomaterial scaffold also play a critical role in ASC differentiation. Histologic and immunohistologic methods for assessing chondrogenesis are also presented. In general, 10-12 weeks are required to assess ASC chondrogenesis in these model systems.
AB - Stem cells can easily be harvested from adipose tissue in large numbers for use in tissue-engineering approaches for cartilage repair or regeneration. In this chapter, we describe in vitro tissue-engineering models that we have used in our laboratory for the chondrogenic induction of adipose-derived stem cells (ASC). In addition to the proper growth factor environment, chondrogenesis requires cells to be maintained in a rounded morphology in three-dimensional (3D) culture, and thus properties of the biomaterial scaffold also play a critical role in ASC differentiation. Histologic and immunohistologic methods for assessing chondrogenesis are also presented. In general, 10-12 weeks are required to assess ASC chondrogenesis in these model systems.
UR - http://www.scopus.com/inward/record.url?scp=79952116092&partnerID=8YFLogxK
U2 - 10.1007/978-1-61737-960-4_15
DO - 10.1007/978-1-61737-960-4_15
M3 - Article
C2 - 21082404
AN - SCOPUS:79952116092
SN - 1064-3745
VL - 702
SP - 201
EP - 217
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -