TY - JOUR
T1 - Three-dimensional arbitrary trajectory scanning photoacoustic microscopy
AU - Yeh, Chenghung
AU - Soetikno, Brian
AU - Hu, Song
AU - Maslov, Konstantin I.
AU - Wang, Lihong V.
N1 - Publisher Copyright:
© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
PY - 2015/4/1
Y1 - 2015/4/1
N2 - We have enhanced photoacoustic microscopy with three-dimensional arbitrary trajectory (3-DAT) scanning, which can rapidly image selected vessels over a large field of view (FOV) and maintain a high signal-to-noise ratio (SNR) despite the depth variation of the vessels. We showed that hemoglobin oxygen saturation (sO2) and blood flow can be measured simultaneously in a mouse ear in vivo at a frame rate 67 times greater than that of a traditional two-dimensional raster scan. We also observed sO2 dynamics in response to switching from systemic hypoxia to hyperoxia. 3-DAT-scanning photoacoustic microscopy. Schematic diagram of the 3D scanning stage and method. Photoacoustic microscopy was enhanced with three-dimensional arbitrary trajectory (3-DAT) scanning, which can rapidly image selected vessels over a large field of view (FOV) and maintain a high signal-tonoise ratio (SNR) despite the depth variation of the vessels. It is shown that hemoglobin oxygen saturation (sO2) and blood flow can be measured simultaneously in a mouse ear in vivo at a frame rate 67 times greater than that of a traditional two-dimensional raster scan. sO2 dynamics were also observed in response to switching from systemic hypoxia to hyperoxia.
AB - We have enhanced photoacoustic microscopy with three-dimensional arbitrary trajectory (3-DAT) scanning, which can rapidly image selected vessels over a large field of view (FOV) and maintain a high signal-to-noise ratio (SNR) despite the depth variation of the vessels. We showed that hemoglobin oxygen saturation (sO2) and blood flow can be measured simultaneously in a mouse ear in vivo at a frame rate 67 times greater than that of a traditional two-dimensional raster scan. We also observed sO2 dynamics in response to switching from systemic hypoxia to hyperoxia. 3-DAT-scanning photoacoustic microscopy. Schematic diagram of the 3D scanning stage and method. Photoacoustic microscopy was enhanced with three-dimensional arbitrary trajectory (3-DAT) scanning, which can rapidly image selected vessels over a large field of view (FOV) and maintain a high signal-tonoise ratio (SNR) despite the depth variation of the vessels. It is shown that hemoglobin oxygen saturation (sO2) and blood flow can be measured simultaneously in a mouse ear in vivo at a frame rate 67 times greater than that of a traditional two-dimensional raster scan. sO2 dynamics were also observed in response to switching from systemic hypoxia to hyperoxia.
KW - 3-D arbitrary trajectory scanning
KW - Hemoglobin oxygen saturation
KW - Metabolic rate of oxygen
KW - Optical-resolution photoacoustic microscopy
KW - Raster scanning
UR - https://www.scopus.com/pages/publications/84926176049
U2 - 10.1002/jbio.201400055
DO - 10.1002/jbio.201400055
M3 - Letter
C2 - 25077689
AN - SCOPUS:84926176049
SN - 1864-063X
VL - 8
SP - 303
EP - 308
JO - Journal of Biophotonics
JF - Journal of Biophotonics
IS - 4
ER -