TY - JOUR
T1 - Three-dimensional anatomical characterization of the developing mouse brain by diffusion tensor microimaging
AU - Zhang, Jiangyang
AU - Richards, Linda J.
AU - Yarowsky, Paul
AU - Huang, Hao
AU - Van Zijl, Peter C.M.
AU - Mori, Susumu
N1 - Funding Information:
This study was supported by NIH grants RO1 AG20012-01, RO3 HD41407-01A1, and P41 RR15241-01. We thank Kimberly M. Valentino and Celine Plachez for help in preparing brain specimens.
PY - 2003/11
Y1 - 2003/11
N2 - Investigation of three-dimensional (3D) morphometry of developing brains has been hindered by a lack of imaging modalities that can monitor the 3D evolution of various anatomical structures without sectioning and staining processes. In this study, we combined magnetic resonance microimaging and diffusion tensor imaging techniques to accomplish such visualization. The application of this approach to developing mouse embryos revealed that it could clearly delineate early critical structures such as neuroepithelium, cortical plate, and various axonal structures, and follow their developmental evolution. The technique was applied to the study of the Netrin-1 mutant, allowing verification of its anatomical phenotype.
AB - Investigation of three-dimensional (3D) morphometry of developing brains has been hindered by a lack of imaging modalities that can monitor the 3D evolution of various anatomical structures without sectioning and staining processes. In this study, we combined magnetic resonance microimaging and diffusion tensor imaging techniques to accomplish such visualization. The application of this approach to developing mouse embryos revealed that it could clearly delineate early critical structures such as neuroepithelium, cortical plate, and various axonal structures, and follow their developmental evolution. The technique was applied to the study of the Netrin-1 mutant, allowing verification of its anatomical phenotype.
UR - http://www.scopus.com/inward/record.url?scp=0345415035&partnerID=8YFLogxK
U2 - 10.1016/S1053-8119(03)00410-5
DO - 10.1016/S1053-8119(03)00410-5
M3 - Article
C2 - 14642474
AN - SCOPUS:0345415035
SN - 1053-8119
VL - 20
SP - 1639
EP - 1648
JO - NeuroImage
JF - NeuroImage
IS - 3
ER -