Thin layer chromatography-based assay of O6-methylguanine-DNA methyltransferase activity in tissue

Cliff Robinson, Juan Palomo, Michael A. Vogelbaum

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Expression of the DNA repair enzyme O6-methylguanine-DNA methyltransferase (MGMT) in tumor correlates with resistance to alkylating agents, and depletion of MGMT activity can enhance chemotherapy-induced tumor cytotoxicity. The most common assays of MGMT activity are time-consuming and employ radioactivity. The assay described here uses a fluorescently labeled O6-benzylguanine derivative in conjunction with thin layer chromatography to eliminate the use of radioactivity and allows MGMT activity to be rapidly measured in minimally prepared cell or tissue extracts.

Original languageEnglish
Pages (from-to)263-265
Number of pages3
JournalAnalytical Biochemistry
Volume405
Issue number2
DOIs
StatePublished - Oct 2010

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