Thermodynamically coupled biosensors for detecting neutralizing antibodies against SARS-CoV-2 variants

Jason Z. Zhang; Hsien Wei Yeh; Alexandra C. Walls; Basile I.M. Wicky; Kaitlin R. Sprouse; Laura A. VanBlargan; Rebecca Treger; Alfredo Quijano-Rubio; Minh N. Pham; John C. Kraft; Ian C. Haydon; Wei Yang; Michelle DeWitt; John E. Bowen; Cameron M. Chow; Lauren Carter; Rashmi Ravichandran; Mark H. Wener; Lance Stewart; David Veesler; Michael S. Diamond; Alexander L. Greninger; David M. Koelle; David Baker

doi:10.1038/s41587-022-01280-8

Thermodynamically coupled biosensors for detecting neutralizing antibodies against SARS-CoV-2 variants

Jason Z. Zhang
, Hsien Wei Yeh
, Alexandra C. Walls
, Basile I.M. Wicky
, Kaitlin R. Sprouse
, Laura A. VanBlargan
, Rebecca Treger
, Alfredo Quijano-Rubio
, Minh N. Pham
, John C. Kraft
, Ian C. Haydon
, Wei Yang
, Michelle DeWitt
, John E. Bowen
, Cameron M. Chow
, Lauren Carter
, Rashmi Ravichandran
, Mark H. Wener
, Lance Stewart
, David Veesler

Michael S. Diamond, Alexander L. Greninger, David M. Koelle, David Baker

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

We designed a protein biosensor that uses thermodynamic coupling for sensitive and rapid detection of neutralizing antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants in serum. The biosensor is a switchable, caged luciferase–receptor-binding domain (RBD) construct that detects serum-antibody interference with the binding of virus RBD to angiotensin-converting enzyme 2 (ACE-2) as a proxy for neutralization. Our coupling approach does not require target modification and can better distinguish sample-to-sample differences in analyte binding affinity and abundance than traditional competition-based assays.

Original language	English
Pages (from-to)	1336-1340
Number of pages	5
Journal	Nature Biotechnology
Volume	40
Issue number	9
DOIs	https://doi.org/10.1038/s41587-022-01280-8
State	Published - Sep 2022

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Zhang, J. Z., Yeh, H. W., Walls, A. C., Wicky, B. I. M., Sprouse, K. R., VanBlargan, L. A., Treger, R., Quijano-Rubio, A., Pham, M. N., Kraft, J. C., Haydon, I. C., Yang, W., DeWitt, M., Bowen, J. E., Chow, C. M., Carter, L., Ravichandran, R., Wener, M. H., Stewart, L., ... Baker, D. (2022). Thermodynamically coupled biosensors for detecting neutralizing antibodies against SARS-CoV-2 variants. Nature Biotechnology, 40(9), 1336-1340. https://doi.org/10.1038/s41587-022-01280-8

@article{6ca084d4d3f14c16911fcd22d1200b35,

title = "Thermodynamically coupled biosensors for detecting neutralizing antibodies against SARS-CoV-2 variants",

abstract = "We designed a protein biosensor that uses thermodynamic coupling for sensitive and rapid detection of neutralizing antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants in serum. The biosensor is a switchable, caged luciferase–receptor-binding domain (RBD) construct that detects serum-antibody interference with the binding of virus RBD to angiotensin-converting enzyme 2 (ACE-2) as a proxy for neutralization. Our coupling approach does not require target modification and can better distinguish sample-to-sample differences in analyte binding affinity and abundance than traditional competition-based assays.",

author = "Zhang, \{Jason Z.\} and Yeh, \{Hsien Wei\} and Walls, \{Alexandra C.\} and Wicky, \{Basile I.M.\} and Sprouse, \{Kaitlin R.\} and VanBlargan, \{Laura A.\} and Rebecca Treger and Alfredo Quijano-Rubio and Pham, \{Minh N.\} and Kraft, \{John C.\} and Haydon, \{Ian C.\} and Wei Yang and Michelle DeWitt and Bowen, \{John E.\} and Chow, \{Cameron M.\} and Lauren Carter and Rashmi Ravichandran and Wener, \{Mark H.\} and Lance Stewart and David Veesler and Diamond, \{Michael S.\} and Greninger, \{Alexander L.\} and Koelle, \{David M.\} and David Baker",

note = "Publisher Copyright: {\textcopyright} 2022, The Author(s).",

year = "2022",

month = sep,

doi = "10.1038/s41587-022-01280-8",

language = "English",

volume = "40",

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journal = "Nature Biotechnology",

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Zhang, JZ, Yeh, HW, Walls, AC, Wicky, BIM, Sprouse, KR, VanBlargan, LA, Treger, R, Quijano-Rubio, A, Pham, MN, Kraft, JC, Haydon, IC, Yang, W, DeWitt, M, Bowen, JE, Chow, CM, Carter, L, Ravichandran, R, Wener, MH, Stewart, L, Veesler, D, Diamond, MS, Greninger, AL, Koelle, DM & Baker, D 2022, 'Thermodynamically coupled biosensors for detecting neutralizing antibodies against SARS-CoV-2 variants', Nature Biotechnology, vol. 40, no. 9, pp. 1336-1340. https://doi.org/10.1038/s41587-022-01280-8

TY - JOUR

T1 - Thermodynamically coupled biosensors for detecting neutralizing antibodies against SARS-CoV-2 variants

AU - Zhang, Jason Z.

AU - Yeh, Hsien Wei

AU - Walls, Alexandra C.

AU - Wicky, Basile I.M.

AU - Sprouse, Kaitlin R.

AU - VanBlargan, Laura A.

AU - Treger, Rebecca

AU - Quijano-Rubio, Alfredo

AU - Pham, Minh N.

AU - Kraft, John C.

AU - Haydon, Ian C.

AU - Yang, Wei

AU - DeWitt, Michelle

AU - Bowen, John E.

AU - Chow, Cameron M.

AU - Carter, Lauren

AU - Ravichandran, Rashmi

AU - Wener, Mark H.

AU - Stewart, Lance

AU - Veesler, David

AU - Diamond, Michael S.

AU - Greninger, Alexander L.

AU - Koelle, David M.

AU - Baker, David

PY - 2022/9

Y1 - 2022/9

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AB - We designed a protein biosensor that uses thermodynamic coupling for sensitive and rapid detection of neutralizing antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants in serum. The biosensor is a switchable, caged luciferase–receptor-binding domain (RBD) construct that detects serum-antibody interference with the binding of virus RBD to angiotensin-converting enzyme 2 (ACE-2) as a proxy for neutralization. Our coupling approach does not require target modification and can better distinguish sample-to-sample differences in analyte binding affinity and abundance than traditional competition-based assays.

UR - https://www.scopus.com/pages/publications/85128979650

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DO - 10.1038/s41587-022-01280-8

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VL - 40

SP - 1336

EP - 1340

JO - Nature Biotechnology

JF - Nature Biotechnology

IS - 9

ER -

Thermodynamically coupled biosensors for detecting neutralizing antibodies against SARS-CoV-2 variants

Abstract

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