Thermodynamic Methods for Model-Independent Determination of Equilibrium Binding Isotherms for Protein-DNA Interactions: Spectroscopic Approaches to Monitor Binding

Timothy M. Lohman; Wlodzimierz Bujalowski

doi:10.1016/0076-6879(91)08017-C

Thermodynamic Methods for Model-Independent Determination of Equilibrium Binding Isotherms for Protein-DNA Interactions: Spectroscopic Approaches to Monitor Binding

Timothy M. Lohman
, Wlodzimierz Bujalowski

Research output: Contribution to journal › Article › peer-review

Original language	English
Pages (from-to)	258-290
Number of pages	33
Journal	Methods in enzymology
Volume	208
Issue number	C
DOIs	https://doi.org/10.1016/0076-6879(91)08017-C
State	Published - Jan 1991

Access to Document

10.1016/0076-6879(91)08017-C

Cite this

@article{87ed9eee81ab4a59818cefb8943cb1ba,

title = "Thermodynamic Methods for Model-Independent Determination of Equilibrium Binding Isotherms for Protein-DNA Interactions: Spectroscopic Approaches to Monitor Binding",

author = "Lohman, \{Timothy M.\} and Wlodzimierz Bujalowski",

note = "Funding Information: We thank Dave Mascotti, Nick Pace, Dave Giedroc, and Don Pettigrew for comments on the manuscripta nd Lisa Lohmanf or help in preparing the figures. The preparation of this chapter was supported by the National Instituteso f Health (GM30498, GM39062), the Robert A. Welch Foundation (A-898), and the Texas Agricultural Experiment Station. T.M.L. is the recipient of an American Cancer Society Faculty Research Award (FRA-303). Funding Information: This work was supported by grants from INSERM (No. 88-1003) and FRM. The CNRS has specifically financed the cost of development of the mixing device described in the section, Rapid Mixing. J.G. is a recipient of a long-term EMBO fellowship.",

year = "1991",

month = jan,

doi = "10.1016/0076-6879(91)08017-C",

language = "English",

volume = "208",

pages = "258--290",

journal = "Methods in enzymology",

issn = "0076-6879",

number = "C",

}

TY - JOUR

T1 - Thermodynamic Methods for Model-Independent Determination of Equilibrium Binding Isotherms for Protein-DNA Interactions

T2 - Spectroscopic Approaches to Monitor Binding

AU - Lohman, Timothy M.

AU - Bujalowski, Wlodzimierz

N1 - Funding Information: We thank Dave Mascotti, Nick Pace, Dave Giedroc, and Don Pettigrew for comments on the manuscripta nd Lisa Lohmanf or help in preparing the figures. The preparation of this chapter was supported by the National Instituteso f Health (GM30498, GM39062), the Robert A. Welch Foundation (A-898), and the Texas Agricultural Experiment Station. T.M.L. is the recipient of an American Cancer Society Faculty Research Award (FRA-303). Funding Information: This work was supported by grants from INSERM (No. 88-1003) and FRM. The CNRS has specifically financed the cost of development of the mixing device described in the section, Rapid Mixing. J.G. is a recipient of a long-term EMBO fellowship.

PY - 1991/1

Y1 - 1991/1

UR - https://www.scopus.com/pages/publications/0026351773

U2 - 10.1016/0076-6879(91)08017-C

DO - 10.1016/0076-6879(91)08017-C

M3 - Article

C2 - 1779838

AN - SCOPUS:0026351773

SN - 0076-6879

VL - 208

SP - 258

EP - 290

JO - Methods in enzymology

JF - Methods in enzymology

IS - C

ER -

Thermodynamic Methods for Model-Independent Determination of Equilibrium Binding Isotherms for Protein-DNA Interactions: Spectroscopic Approaches to Monitor Binding

Access to Document

Other files and links

Cite this