Thermodynamic Methods for Model-Independent Determination of Equilibrium Binding Isotherms for Protein-DNA Interactions: Spectroscopic Approaches to Monitor Binding

Timothy M. Lohman; Wlodzimierz Bujalowski

doi:10.1016/0076-6879(91)08017-C

Thermodynamic Methods for Model-Independent Determination of Equilibrium Binding Isotherms for Protein-DNA Interactions: Spectroscopic Approaches to Monitor Binding

Timothy M. Lohman, Wlodzimierz Bujalowski

Research output: Contribution to journal › Article › peer-review

Original language	English
Pages (from-to)	258-290
Number of pages	33
Journal	Methods in enzymology
Volume	208
Issue number	C
DOIs	https://doi.org/10.1016/0076-6879(91)08017-C
State	Published - Jan 1991

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10.1016/0076-6879(91)08017-C

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title = "Thermodynamic Methods for Model-Independent Determination of Equilibrium Binding Isotherms for Protein-DNA Interactions: Spectroscopic Approaches to Monitor Binding",

author = "Lohman, {Timothy M.} and Wlodzimierz Bujalowski",

note = "Funding Information: We thank Dave Mascotti, Nick Pace, Dave Giedroc, and Don Pettigrew for comments on the manuscripta nd Lisa Lohmanf or help in preparing the figures. The preparation of this chapter was supported by the National Instituteso f Health (GM30498, GM39062), the Robert A. Welch Foundation (A-898), and the Texas Agricultural Experiment Station. T.M.L. is the recipient of an American Cancer Society Faculty Research Award (FRA-303). Funding Information: This work was supported by grants from INSERM (No. 88-1003) and FRM. The CNRS has specifically financed the cost of development of the mixing device described in the section, Rapid Mixing. J.G. is a recipient of a long-term EMBO fellowship.",

year = "1991",

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AU - Lohman, Timothy M.

AU - Bujalowski, Wlodzimierz

N1 - Funding Information: We thank Dave Mascotti, Nick Pace, Dave Giedroc, and Don Pettigrew for comments on the manuscripta nd Lisa Lohmanf or help in preparing the figures. The preparation of this chapter was supported by the National Instituteso f Health (GM30498, GM39062), the Robert A. Welch Foundation (A-898), and the Texas Agricultural Experiment Station. T.M.L. is the recipient of an American Cancer Society Faculty Research Award (FRA-303). Funding Information: This work was supported by grants from INSERM (No. 88-1003) and FRM. The CNRS has specifically financed the cost of development of the mixing device described in the section, Rapid Mixing. J.G. is a recipient of a long-term EMBO fellowship.

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Thermodynamic Methods for Model-Independent Determination of Equilibrium Binding Isotherms for Protein-DNA Interactions: Spectroscopic Approaches to Monitor Binding

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