Thermodynamic characterization of RNA triloops

Praneetha Thulasi, Lopa K. Pandya, Brent M. Znosko

Research output: Contribution to journalArticlepeer-review

18 Scopus citations


Relatively few thermodynamic parameters are available for RNA triloops. Therefore, 24 stem-loop sequences containing naturally occurring triloops were optically melted, and the thermodynamic parameters ΔH°, ΔS°, ΔG°37, and TM for each stem-loop were determined. These new experimental values, on average, are 0.5 kcal/mol different from the values predicted for these triloops using the model proposed by Mathews et al. [Mathews, D. H., Disney, M. D., Childs, J. L., Schroeder, S. J., Zuker, M., and Turner, D. H. (2004) Proc. Natl. Acad. Sci. U.S.A. 101, 7287-7292]. The data for the 24 triloops reported here were then combined with the data for five triloops that were published previously. A new model was derived to predict the free energy contribution of previously unmeasured triloops. The average absolute difference between the measured values and the values predicted using this proposed model is 0.3 kcal/mol. These new experimental data and updated predictive model allow for more accurate calculations of the free energy of RNA stem-loops containing triloops and, furthermore, should allow for improved prediction of secondary structure from sequence.

Original languageEnglish
Pages (from-to)9058-9062
Number of pages5
Issue number42
StatePublished - Oct 26 2010


Dive into the research topics of 'Thermodynamic characterization of RNA triloops'. Together they form a unique fingerprint.

Cite this