TY - JOUR
T1 - Therapeutic efficacy as predicted by quantitative assessment of murine RIF-1 tumour pH and phosphorous metabolite response during hyperthermia
T2 - An in vivo 31P NMR study
AU - Bezabeh, T.
AU - Evelhoch, J. L.
AU - Thompson, P.
AU - Sloop, D. J.
AU - Ackerman, J. J.H.
N1 - Funding Information:
This work was supported through grants from the National Cancer Institute: RO1 CA-40411, R24 CA-83060 (Small Animal Imaging Research Program), and P30 CA91842 (Cancer Center Support). The assistance of Dr G. Larry Bretthorst in the area of Bayesian analysis of time domain NMR signals is greatly appreciated. This investigation is described in additional detail in the PhD thesis (Washington University, St. Louis, 1993) of Tedros Bezabeh.
PY - 2004/6
Y1 - 2004/6
N2 - Described herein are the initial findings from an 'in-magnet' 31P NMR compatible hyperthermia system capable of concurrently heating and monitoring the metabolic response of murine tumours; the murine radiation induced fibrosarcoma (RIF-1) was employed for these studies. At thermal doses sufficient to raise tumour temperature to 41.5 and 43°C for a period of 30 min, a marked and rapid decrease in nucleoside triphosphate concentration and in pH was observed during the heating period, while inorganic phosphate concentration increased significantly but more gradually. These 31P NMR determined metabolic indices remained depressed/elevated throughout a 1.5 h post-hyperthermia monitoring period. Importantly, these metabolic indices correlated significantly with specific growth delay. This suggests a possible role for NMR spectroscopy in early assessment, and perhaps control, of therapeutic response to hyperthermia.
AB - Described herein are the initial findings from an 'in-magnet' 31P NMR compatible hyperthermia system capable of concurrently heating and monitoring the metabolic response of murine tumours; the murine radiation induced fibrosarcoma (RIF-1) was employed for these studies. At thermal doses sufficient to raise tumour temperature to 41.5 and 43°C for a period of 30 min, a marked and rapid decrease in nucleoside triphosphate concentration and in pH was observed during the heating period, while inorganic phosphate concentration increased significantly but more gradually. These 31P NMR determined metabolic indices remained depressed/elevated throughout a 1.5 h post-hyperthermia monitoring period. Importantly, these metabolic indices correlated significantly with specific growth delay. This suggests a possible role for NMR spectroscopy in early assessment, and perhaps control, of therapeutic response to hyperthermia.
KW - Bayesian analysis
KW - Specific growth delay
KW - Surviving fraction
KW - Therapeutic response
KW - Thermal dose
UR - http://www.scopus.com/inward/record.url?scp=3042635933&partnerID=8YFLogxK
U2 - 10.1080/0265673042000196469
DO - 10.1080/0265673042000196469
M3 - Review article
C2 - 15204517
AN - SCOPUS:3042635933
SN - 0265-6736
VL - 20
SP - 335
EP - 357
JO - International Journal of Hyperthermia
JF - International Journal of Hyperthermia
IS - 4
ER -