TY - JOUR
T1 - The Zebrafish Glypican Knypek Controls Cell Polarity during Gastrulation Movements of Convergent Extension
AU - Topczewski, Jacek
AU - Sepich, Diane S.
AU - Myers, Dina C.
AU - Walker, Charline
AU - Amores, Angel
AU - Lele, Zsolt
AU - Hammerschmidt, Matthias
AU - Postlethwait, John
AU - Solnica-Krezel, Lilianna
N1 - Funding Information:
We thank B. Appel, D. Greenstein, C. Kimmel, A. Schier, C. Wright, and members of our laboratories for critical discussions and comments on the manuscript. We acknowledge B. Heher and J. Clanton for excellent fish care. We are grateful to Dr. E. Raz for the gift of kny m818 fish and S. Anders, M. Ekker, P. Ingham, H. Takeda, and K. and B. Thisse for cDNA clones, D. Kimelman for cDNA library, and M. Halpern for γ-ray mutagenized males. Confocal experiments were performed in part through the use of the VUMC Cell Imaging Core Resource (supported by National Institutes of Health [NIH] grants CA68485 and DK20593). C.W. was supported by NIH grant HD22486. This work was supported by NIH grant GM55101 to L.S.K., who is a Pew Scholar in the Biomedical Sciences.
PY - 2001/8
Y1 - 2001/8
N2 - Mutations in the zebrafish knypek locus impair gastrulation movements of convergent extension that narrow embryonic body and elongate it from head to tail. We demonstrate that knypek regulates cellular movements but not cell fate specification. Convergent extension movement defects in knypek are associated with abnormal cell polarity, as mutant cells fail to elongate and align medio-laterally. Positional cloning reveals that knypek encodes a member of the glypican family of heparan sulfate proteoglycans. Double mutant and overexpression analyses show that Knypek potentiates Wnt11 signaling, mediating convergent extension. These studies provide experimental and genetic evidence that glypican Knypek acts during vertebrate gastrulation as a positive modulator of non-canonical Wnt signaling to establish polarized cell behaviors underlying convergent extension movements.
AB - Mutations in the zebrafish knypek locus impair gastrulation movements of convergent extension that narrow embryonic body and elongate it from head to tail. We demonstrate that knypek regulates cellular movements but not cell fate specification. Convergent extension movement defects in knypek are associated with abnormal cell polarity, as mutant cells fail to elongate and align medio-laterally. Positional cloning reveals that knypek encodes a member of the glypican family of heparan sulfate proteoglycans. Double mutant and overexpression analyses show that Knypek potentiates Wnt11 signaling, mediating convergent extension. These studies provide experimental and genetic evidence that glypican Knypek acts during vertebrate gastrulation as a positive modulator of non-canonical Wnt signaling to establish polarized cell behaviors underlying convergent extension movements.
UR - http://www.scopus.com/inward/record.url?scp=0035434453&partnerID=8YFLogxK
U2 - 10.1016/S1534-5807(01)00005-3
DO - 10.1016/S1534-5807(01)00005-3
M3 - Article
C2 - 11702784
AN - SCOPUS:0035434453
SN - 1534-5807
VL - 1
SP - 251
EP - 264
JO - Developmental cell
JF - Developmental cell
IS - 2
ER -