The vitronectin receptor serves as an accessory molecule for the activation of a subset of γ/δ T cells

K. Roberts, W. M. Yokoyama, P. J. Kehn, E. M. Shevach

Research output: Contribution to journalArticle

85 Scopus citations

Abstract

Constitutive production of cytokines was observed in 3 of 12 γ/δ T cell lines derived from murine epidermis and correlated with the expression of the Cγ4, Vδ6 T cell receptor (TCR). After adaptation of one of the lines (T195/BW) to serum-free culture conditions, cessation of the 'spontaneous' production of interleukin 4 (IL-4) was observed and IL-4 production could then be induced by the addition of RGD-containing extracellular matrix (ECM) proteins to the culture. The response to the ECM proteins could be completely inhibited by a mAb to the murine vitronectin receptor (VNR). However, the induction of IL-4 production could also be inhibited by anti-CD3 and by an anti-clonotypic mAb to the TCR-γ/δ of T195/BW. As TCR-γ/δ loss mutants of T195/BW also failed to respond to ECM proteins, these data demonstrate that engagement of the VNR by its ligand is necessary, but not sufficient, for the induction of IL-4 production. Furthermore, the VNR is expressed by many other T cell clones (both γ/δ and α/β), none of which produce lymphokines constitutively. Taken together, these observations strongly favor the view that not only is coexpression of the VNR and TCR required for the induction of IL-4 production, but that the TCR must also be engaged by its ligand, most likely a cell surface antigen expressed by the hybridoma itself.

Original languageEnglish
Pages (from-to)231-240
Number of pages10
JournalJournal of Experimental Medicine
Volume173
Issue number1
DOIs
StatePublished - Jan 25 1991
Externally publishedYes

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