The vesicular transport protein Cgp1p/Vps54p/Tcs3p/Luv1p is required for the integrity of the actin cytoskeleton

T. A. Fiedler, T. S. Karpova, U. Fleig, M. E. Young, J. A. Cooper, Johannes H. Hegemann

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The CGP1 gene was identified in a screen for mutations that were synthetic lethal in combination with a deletion of the gene (CPF1) for centromere and promoter factor 1. Cells deleted for CGP1 showed reduced viability, were temperature sensitive for growth and exhibited altered sensitivity to microtubule-destabilizing drugs. Furthermore, Δcgp1 cells showed increased rates of loss of a circular minichromosome and defects in the positioning of the short mitotic spindle. Further phenotypic analysis of Δcgp1 cells revealed that loss of Cgp1p function led to severe depolarization of the actin cytoskeleton. In addition, cells deleted for CGP1 were hypersensitive to the actin-disrupting compound Latrunculin-A, exhibited strongly reduced polarized localization of the unconventional myosin Myo2p, and showed defects in other actin-related processes, such as shmoo formation and cell wall integrity. Cgp1p was recently identified by several groups as Vps54p, which is a member of the VFT complex that is involved in vesicular protein transport at the level of the late Golgi, acting as a tethering factor. Our data show for the first time that Cgp1p/Vps54p links aspects of vesicular protein transport with the organization of the actin cytoskeleton.

Original languageEnglish
Pages (from-to)190-205
Number of pages16
JournalMolecular Genetics and Genomics
Volume268
Issue number2
DOIs
StatePublished - 2002

Keywords

  • Actin cytoskeleton
  • CPF1
  • Chromosome segregation
  • Saccharomyces cerevisiae
  • Vesicular protein trafficking

Fingerprint

Dive into the research topics of 'The vesicular transport protein Cgp1p/Vps54p/Tcs3p/Luv1p is required for the integrity of the actin cytoskeleton'. Together they form a unique fingerprint.

Cite this