The use of antisense oligonucleotides in evaluating survivin as a therapeutic target for radiation sensitization in lung cancer

Eric T. Shinohara, Dennis E. Hallahan, Bo Lu

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Elucidating the mechanism of over and under expression of proteins is critical in developing a better understanding of cancer. Multiple techniques are used to examine differential expression of proteins in cells and assess changes in protein expression in response to therapies such as radiation. Reduced expression can be caused by protein inactivation, mRNA instability, or reduced transcription. The following protocol was used to determine the mechanism for the reduced expression of an antiapoptotic factor, survivin, in normal tissues in response to radiation and the defect in cancer cells that prevents this reduction. We also examined ways to overcome survivin over expression in cancer cells in order to sensitize them to radiation. We will focus on the use of antisense oligonucleotides, cell cycle analysis, and luciferase reporter genes.

Original languageEnglish
Pages (from-to)250-256
Number of pages7
JournalBiological Procedures Online
Volume6
Issue number1
DOIs
StatePublished - Oct 25 2004
Externally publishedYes

Keywords

  • Antisense oligonucleotides
  • Luciferase
  • Survivin

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