TY - JOUR
T1 - The translation of a human placental lactogen mRNA fraction in heterologous cell-free systems
T2 - The synthesis of a possible precursor
AU - Boime, Irving
AU - Boguslawski, Sophie
AU - Caine, Jean
N1 - Funding Information:
We are grateful to Mrs. Marion Nau and Dr. P. Leder for helping us with the wheat germ cell-free system. This work was aided in part by a grant from the Population Council (#M73, 135) and by a Departmental Grant from the Ford Foundation.
PY - 1975/1/6
Y1 - 1975/1/6
N2 - RNA extracted from term placental homogenates directed the synthesis of human placental lactogen (HPL) in a heterologous cell-free system derived from Krebs II ascites tumor cells. HPL was one of the major products synthesized. It was identified on SDS-polyacrylamide gels and by trypsin fingerprinting. Translation of placental RNA in a cell-free system from wheat germ resulted in the synthesis of a protein that migrated slower than HPL on SDS-polyacrylamide gels; no labeled protein was observed that comigrated with authentic HPL. This heavier protein had a molecular weight of about 25,000. It contained the same overlapping HPL tryptic peptides seen when the RNA was translated in the ascites cell-free system. This raises the possibility that a HPL precursor was synthesized in the wheat germ system.
AB - RNA extracted from term placental homogenates directed the synthesis of human placental lactogen (HPL) in a heterologous cell-free system derived from Krebs II ascites tumor cells. HPL was one of the major products synthesized. It was identified on SDS-polyacrylamide gels and by trypsin fingerprinting. Translation of placental RNA in a cell-free system from wheat germ resulted in the synthesis of a protein that migrated slower than HPL on SDS-polyacrylamide gels; no labeled protein was observed that comigrated with authentic HPL. This heavier protein had a molecular weight of about 25,000. It contained the same overlapping HPL tryptic peptides seen when the RNA was translated in the ascites cell-free system. This raises the possibility that a HPL precursor was synthesized in the wheat germ system.
UR - https://www.scopus.com/pages/publications/0016436536
U2 - 10.1016/S0006-291X(75)80411-6
DO - 10.1016/S0006-291X(75)80411-6
M3 - Article
C2 - 1111509
AN - SCOPUS:0016436536
SN - 0006-291X
VL - 62
SP - 103
EP - 109
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -