TY - JOUR
T1 - The transcriptional corepressor NAB2 blocks Egr-1-mediated growth factor activation and angiogenesis
AU - Houston, Parul
AU - Campbell, Callum J.
AU - Svaren, John
AU - Milbrandt, Jeffrey
AU - Braddock, Martin
PY - 2001
Y1 - 2001
N2 - Effective tissue repair results from a rapid, temporally orchestrated series of events. At the site of local tissue injury, the production of many growth factors and cytokines is, in part, stimulated by the early growth response transcription factors such as Egr-1. Egr-1 protein binds to a family of corepressor proteins called NAB which function to block or limit Egr-1 trans-activation of cognate target genes. NAB2 blocks Egr-1 activation of the tissue factor (TF) promoter, Egr-1 stimulated production of PDGF-AB, HGF, TGFβ, and VEGF and the endogenous expression of PDGF-AB and TGFβ. Expression of a wild-type NAB2 but not a dominant negative NAB2 mutant abrogates Egr-1 driven TF promoter activity and tubule formation in an in vitro model of angiogenesis. These findings may have importance in any tissue that is subject to scarring after acute or chronic injury.
AB - Effective tissue repair results from a rapid, temporally orchestrated series of events. At the site of local tissue injury, the production of many growth factors and cytokines is, in part, stimulated by the early growth response transcription factors such as Egr-1. Egr-1 protein binds to a family of corepressor proteins called NAB which function to block or limit Egr-1 trans-activation of cognate target genes. NAB2 blocks Egr-1 activation of the tissue factor (TF) promoter, Egr-1 stimulated production of PDGF-AB, HGF, TGFβ, and VEGF and the endogenous expression of PDGF-AB and TGFβ. Expression of a wild-type NAB2 but not a dominant negative NAB2 mutant abrogates Egr-1 driven TF promoter activity and tubule formation in an in vitro model of angiogenesis. These findings may have importance in any tissue that is subject to scarring after acute or chronic injury.
KW - Angiogenesis
KW - Egr-1
KW - Gene therapy
KW - Growth factor
KW - NAB2
KW - Tissue repair
KW - Transcriptional repressor
UR - http://www.scopus.com/inward/record.url?scp=0034810154&partnerID=8YFLogxK
U2 - 10.1006/bbrc.2001.4810
DO - 10.1006/bbrc.2001.4810
M3 - Article
C2 - 11327726
AN - SCOPUS:0034810154
SN - 0006-291X
VL - 283
SP - 480
EP - 486
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -