TY - JOUR
T1 - The T cell leukemia oncoprotein SCL/tal-1 is essential for development of all hematopoietic lineages
AU - Porcher, Catherine
AU - Swat, Wojciech
AU - Rockwell, Karen
AU - Fujiwara, Yuko
AU - Alt, Frederick W.
AU - Orkin, Stuart H.
N1 - Funding Information:
Correspondence should be addressed to S. H. O. C. P. is grateful to Mitchell Weiss for advice on in vitro ES cell differentiation. We thank Robert Hawley for the MSCV retrovirus plasmid, Michelle Kelliher for wild-type SCL cDNA, and Laurie Davidson for injections of RAG-2−/− blastocysts. C. P. was funded in part by the Institut National de la Santé et de la Recherche Médicale (INSERM, France). F. W. A. and S. H. O. are Investigators of the Howard Hughes Medical Institute.
PY - 1996/7/12
Y1 - 1996/7/12
N2 - The T cell leukemia oncoprotein SCL/tal-1, a basic-helix-loop-helix transcription factor, is required for production of embryonic red blood cells in the mouse yolk sac. To define roles in other lineages, we studied the hematopoietic potential of homozygous mutant SCL/tal- -/- embryonic stem cells upon in vitro differentiation and in vivo in chimeric mice. Here we show that in the absence of SCL/tal-1, hematopoiesis, including the generation of red cells, myeloid cells, megakaryocytes, mast cells, and both T and B lymphoid cells, is undetectable. These findings suggest that SCL/tal- 1 functions very early in hematopoietic development, either in specification of ventral mesoderm to a blood cell fate, or in formation or maintenance of immature progenitors.
AB - The T cell leukemia oncoprotein SCL/tal-1, a basic-helix-loop-helix transcription factor, is required for production of embryonic red blood cells in the mouse yolk sac. To define roles in other lineages, we studied the hematopoietic potential of homozygous mutant SCL/tal- -/- embryonic stem cells upon in vitro differentiation and in vivo in chimeric mice. Here we show that in the absence of SCL/tal-1, hematopoiesis, including the generation of red cells, myeloid cells, megakaryocytes, mast cells, and both T and B lymphoid cells, is undetectable. These findings suggest that SCL/tal- 1 functions very early in hematopoietic development, either in specification of ventral mesoderm to a blood cell fate, or in formation or maintenance of immature progenitors.
UR - http://www.scopus.com/inward/record.url?scp=0030581174&partnerID=8YFLogxK
U2 - 10.1016/S0092-8674(00)80076-8
DO - 10.1016/S0092-8674(00)80076-8
M3 - Article
C2 - 8689686
AN - SCOPUS:0030581174
VL - 86
SP - 47
EP - 57
JO - Cell
JF - Cell
SN - 0092-8674
IS - 1
ER -