The synthesis of sulfated dextran beads for isolation of human plasma coagulation factors II, IX, and X

Joseph P. Miletich; George J. Broze; Philip W. Majerus

doi:10.1016/0003-2697(80)90462-5

The synthesis of sulfated dextran beads for isolation of human plasma coagulation factors II, IX, and X

Joseph P. Miletich
, George J. Broze
, Philip W. Majerus

Division of Hematology

Research output: Contribution to journal › Article › peer-review

131 Scopus citations

Abstract

We have prepared a new matrix for the chromatography of coagulation factors using bead-polymerized dextran (Sephadex) sulfated by anhydrous reaction with chlorosulfonic acid. The material is a useful adsorbent for isolation of human plasma coagulation Factors II, IX, and X. Good recoveries (70, 40, and 50% for Factors II, IX, and X, respectively) of proteins homogeneous by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and >99% free of contaminating activities can be obtained from human plasma in 2 days. This new chromatographic material greatly simplifies the isolation of these coagulation factors.

Original language	English
Pages (from-to)	304-310
Number of pages	7
Journal	Analytical Biochemistry
Volume	105
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(80)90462-5
State	Published - Jun 1980

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title = "The synthesis of sulfated dextran beads for isolation of human plasma coagulation factors II, IX, and X",

abstract = "We have prepared a new matrix for the chromatography of coagulation factors using bead-polymerized dextran (Sephadex) sulfated by anhydrous reaction with chlorosulfonic acid. The material is a useful adsorbent for isolation of human plasma coagulation Factors II, IX, and X. Good recoveries (70, 40, and 50\% for Factors II, IX, and X, respectively) of proteins homogeneous by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and >99\% free of contaminating activities can be obtained from human plasma in 2 days. This new chromatographic material greatly simplifies the isolation of these coagulation factors.",

author = "Miletich, \{Joseph P.\} and Broze, \{George J.\} and Majerus, \{Philip W.\}",

note = "Funding Information: 1 This research was supported by Grants HLBI 14147 (Specialized Center in Thrombosis), HL 12820, and HL 16634 from the National Institutes of Health, and in part by NIH Research Service Award GM 07200, Medical Scientist, from the National Institute of General Medical Sciences.",

year = "1980",

month = jun,

doi = "10.1016/0003-2697(80)90462-5",

language = "English",

volume = "105",

pages = "304--310",

journal = "Analytical Biochemistry",

issn = "0003-2697",

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TY - JOUR

T1 - The synthesis of sulfated dextran beads for isolation of human plasma coagulation factors II, IX, and X

AU - Miletich, Joseph P.

AU - Broze, George J.

AU - Majerus, Philip W.

N1 - Funding Information: 1 This research was supported by Grants HLBI 14147 (Specialized Center in Thrombosis), HL 12820, and HL 16634 from the National Institutes of Health, and in part by NIH Research Service Award GM 07200, Medical Scientist, from the National Institute of General Medical Sciences.

PY - 1980/6

Y1 - 1980/6

N2 - We have prepared a new matrix for the chromatography of coagulation factors using bead-polymerized dextran (Sephadex) sulfated by anhydrous reaction with chlorosulfonic acid. The material is a useful adsorbent for isolation of human plasma coagulation Factors II, IX, and X. Good recoveries (70, 40, and 50% for Factors II, IX, and X, respectively) of proteins homogeneous by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and >99% free of contaminating activities can be obtained from human plasma in 2 days. This new chromatographic material greatly simplifies the isolation of these coagulation factors.

AB - We have prepared a new matrix for the chromatography of coagulation factors using bead-polymerized dextran (Sephadex) sulfated by anhydrous reaction with chlorosulfonic acid. The material is a useful adsorbent for isolation of human plasma coagulation Factors II, IX, and X. Good recoveries (70, 40, and 50% for Factors II, IX, and X, respectively) of proteins homogeneous by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and >99% free of contaminating activities can be obtained from human plasma in 2 days. This new chromatographic material greatly simplifies the isolation of these coagulation factors.

UR - https://www.scopus.com/pages/publications/0019127893

U2 - 10.1016/0003-2697(80)90462-5

DO - 10.1016/0003-2697(80)90462-5

M3 - Article

C2 - 6161558

AN - SCOPUS:0019127893

SN - 0003-2697

VL - 105

SP - 304

EP - 310

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 1

ER -

The synthesis of sulfated dextran beads for isolation of human plasma coagulation factors II, IX, and X

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