The synthesis of sulfated dextran beads for isolation of human plasma coagulation factors II, IX, and X

Joseph P. Miletich, George J. Broze, Philip W. Majerus

Research output: Contribution to journalArticle

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Abstract

We have prepared a new matrix for the chromatography of coagulation factors using bead-polymerized dextran (Sephadex) sulfated by anhydrous reaction with chlorosulfonic acid. The material is a useful adsorbent for isolation of human plasma coagulation Factors II, IX, and X. Good recoveries (70, 40, and 50% for Factors II, IX, and X, respectively) of proteins homogeneous by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and >99% free of contaminating activities can be obtained from human plasma in 2 days. This new chromatographic material greatly simplifies the isolation of these coagulation factors.

Original languageEnglish
Pages (from-to)304-310
Number of pages7
JournalAnalytical Biochemistry
Volume105
Issue number1
DOIs
StatePublished - Jun 1980

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