The synaptic vesicle protein SV2 is complexed with an α5-containing laminin on the nerve terminal surface

Young Jin Son, Todd W. Scranton, William J. Sunderland, Sung J. Baek, Jeffrey H. Miner, Joshua R. Sanes, Steven S. Carlson

Research output: Contribution to journalArticle

40 Scopus citations

Abstract

Interactions between growing axons and synaptic basal lamina components direct the formation of neuromuscular junctions during nerve regeneration. Isoforms of laminin containing α5 or β2 chains are potential basal lamina ligands for these interactions. The nerve terminal receptors are unknown. Here we show that SV2, a synaptic vesicle transmembrane proteoglycan, is complexed with a 900-kDa laminin on synaptosomes from the electric organ synapse that is similar to the neuromuscular junctions. Although two laminins are present on synaptosomes, only the 900-kDa laminin is associated with SV2. Other nerve terminal components are absent from this complex. The 900-kDa laminin contains an α5, a β1, and a novel γ chain. To test whether SV2 directly binds the 900-kDa laminin, we looked for interaction between purified SV2 and laminin-1, a laminin isoform with a similar structure. We find SV2 binds with high affinity to purified laminin-1. Our results suggest that a synaptic vesicle component may act as a laminin receptor on the presynaptic plasma membrane; they also suggest a mechanism for activity- dependent adhesion at the synapse.

Original languageEnglish
Pages (from-to)451-460
Number of pages10
JournalJournal of Biological Chemistry
Volume275
Issue number1
DOIs
StatePublished - Jan 7 2000

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