Background: The return of spontaneous circulation (ROSC) is a primary goal of resuscitation. For neonatal resuscitation the International Liaison Committee on Resuscitation (ILCOR) recommends oxygen concentrations ranging from 21% to 100%. Aims and methods: This study (a) compared the efficacy of resuscitation with room air (RA) or 100% O2 in achieving ROSC in 46 neonatal mice with circulatory collapse induced by lethal hypoxia-ischemia (HI) and (b) determined whether re-oxygenation with RA or 100% O2 alters the extent of HI cerebral injury in mice with preserved systemic circulation (n = 31). We also compared changes in generation of reactive oxygen species (ROS) in cerebral mitochondria in response to re-oxygenation with RA or 100% O2. Result: In HI-mice with collapsed circulation re-oxygenation with 100% O2 versus RA resulted in significantly greater rate of ROSC. In HI-mice with preserved systemic circulation and regional (unilateral) cerebral ischemia the restoration of cerebral blood flow was significantly faster upon re-oxygenation with 100% O2, than RA. However, no difference in the extent of brain injury was detected. Regardless of the mode of re-oxygenation, reperfusion in these mice was associated with markedly accelerated ROS production in brain mitochondria. Conclusion: In murine HI associated with circulatory collapse the resuscitation limited to re-oxygenation with 100% O2 is superior to the use of RA in achievement of the ROSC. However, in HI-mice with preserved systemic circulation hyperoxic re-oxygenation has no benefit over the normoxic brain recovery.
|Number of pages||6|
|State||Published - Feb 2010|
- 100% oxygen
- Return of spontaneous circulation
- Room air