The SIOD disorder protein SMARCAL1 is an RPA-interacting protein involved in replication fork restart

Alberto Ciccia, Andrea L. Bredemeyer, Mathew E. Sowa, Marie Emilie Terret, Prasad V. Jallepalli, J. Wade Harper, Stephen J. Elledge

Research output: Contribution to journalArticle

133 Scopus citations

Abstract

The integrity of genomic DNA is continuously challenged by the presence of DNA base lesions or DNA strand breaks. Here we report the identification of a new DNA damage response protein, SMARCAL1 (SWI/SNF-related, matrix associated, actin-dependent regulator of chromatin, subfamily a-like 1), which is a member of the SNF2 family and is mutated in Schimke immunoosseous dysplasia (SIOD). We demonstrate that SMARCAL1 directly interacts with Replication protein A (RPA) and is recruited to sites of DNA damage in an RPA-dependent manner. SMARCAL1-depleted cells display sensitivity to DNA-damaging agents that induce replication fork collapse, and exhibit slower fork recovery and delayed entry into mitosis following S-phase arrest. Furthermore, SIOD patient fibroblasts reconstituted with SMARCAL1 exhibit faster cell cycle progression after S-phase arrest. Thus, the symptoms of SIOD may be caused, at least in part, by defects in the cellular response to DNA replication stress.

Original languageEnglish
Pages (from-to)2415-2425
Number of pages11
JournalGenes and Development
Volume23
Issue number20
DOIs
StatePublished - Oct 15 2009
Externally publishedYes

Keywords

  • DNA double-strand breaks
  • DNA replication
  • DNA replication stress
  • HARP

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    Ciccia, A., Bredemeyer, A. L., Sowa, M. E., Terret, M. E., Jallepalli, P. V., Harper, J. W., & Elledge, S. J. (2009). The SIOD disorder protein SMARCAL1 is an RPA-interacting protein involved in replication fork restart. Genes and Development, 23(20), 2415-2425. https://doi.org/10.1101/gad.1832309