The significance, development and progress of high-throughput combinatorial histone code analysis

Nicolas L. Young, Peter A. DiMaggio, Benjamin A. Garcia

Research output: Contribution to journalReview articlepeer-review

76 Scopus citations

Abstract

The physiological state of eukaryotic DNA is chromatin. Nucleosomes, which consist of DNA in complex with histones, are the fundamental unit of chromatin. The post-translational modifications (PTMs) of histones play a critical role in the control of gene transcription, epigenetics and other DNA-templated processes. It has been known for several years that these PTMs function in concert to allow for the storage and transduction of highly specific signals through combinations of modifications. This code, the combinatorial histone code, functions much like a bar code or combination lock providing the potential for massive information content. The capacity to directly measure these combinatorial histone codes has mostly been laborious and challenging, thus limiting efforts often to one or two samples. Recently, progress has been made in determining such information quickly, quantitatively and sensitively. Here we review both the historical and recent progress toward routine and rapid combinatorial histone code analysis.

Original languageEnglish
Pages (from-to)3983-4000
Number of pages18
JournalCellular and Molecular Life Sciences
Volume67
Issue number23
DOIs
StatePublished - Dec 2010

Keywords

  • Combinatorial
  • Histone
  • Histone code
  • Mass spectrometry
  • Methylation acetylation
  • Modification
  • Proteomic

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