The selective activation of the cardiac sarcolemmal sodium-calcium exchanger by plasmalogenic phosphatidic acid produced by phospholipase D

Calvin C. Hale; Eric G. Ebeling; Fong F. Hsu; David A. Ford

doi:10.1016/S0014-5793(98)00017-9

The selective activation of the cardiac sarcolemmal sodium-calcium exchanger by plasmalogenic phosphatidic acid produced by phospholipase D

Calvin C. Hale, Eric G. Ebeling, Fong F. Hsu, David A. Ford

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22 Scopus citations

Abstract

Since plasmalogens are the predominant phospholipid of cardiac sarcolemma, the activation of the sodium-calcium exchanger by either plasmenylethanolamine or plasmalogenic phosphatidic acid generated by phospholipase D was explored. Sodium-calcium exchange activity was 7-fold greater in proteoliposomes comprised of plasmenylethanolamine compared to proteoliposomes comprised of only plasmenylcholine. Phospholipase D treatment of proteoliposomes resulted in 1 mol % conversion of plasmenylcholine or phosphatidylcholine to their respective phosphatidis acid molecular species with a concomitant 8-fold or 2-fold activation of sodium-calcium exchange activity, respectfully. Thus, phospholipase D-mediated hydrolysis of plasmalogens to phosphatidic acid may be an important mechanism for the regulation of the sodium-calcium exchanger.

Original language	English
Pages (from-to)	247-251
Number of pages	5
Journal	FEBS Letters
Volume	422
Issue number	2
DOIs	https://doi.org/10.1016/S0014-5793(98)00017-9
State	Published - Jan 30 1998

Keywords

Myocardium
Phosphatidic acid
Phospholipase D
Plasmalogen
Sodium-calcium exchange

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10.1016/S0014-5793(98)00017-9

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title = "The selective activation of the cardiac sarcolemmal sodium-calcium exchanger by plasmalogenic phosphatidic acid produced by phospholipase D",

abstract = "Since plasmalogens are the predominant phospholipid of cardiac sarcolemma, the activation of the sodium-calcium exchanger by either plasmenylethanolamine or plasmalogenic phosphatidic acid generated by phospholipase D was explored. Sodium-calcium exchange activity was 7-fold greater in proteoliposomes comprised of plasmenylethanolamine compared to proteoliposomes comprised of only plasmenylcholine. Phospholipase D treatment of proteoliposomes resulted in 1 mol % conversion of plasmenylcholine or phosphatidylcholine to their respective phosphatidis acid molecular species with a concomitant 8-fold or 2-fold activation of sodium-calcium exchange activity, respectfully. Thus, phospholipase D-mediated hydrolysis of plasmalogens to phosphatidic acid may be an important mechanism for the regulation of the sodium-calcium exchanger.",

keywords = "Myocardium, Phosphatidic acid, Phospholipase D, Plasmalogen, Sodium-calcium exchange",

author = "Hale, {Calvin C.} and Ebeling, {Eric G.} and Hsu, {Fong F.} and Ford, {David A.}",

note = "Funding Information: This research was supported by the American Heart Association (C.C.H.), HL 42665 from NIH (D.A.F.), Research Career Development Award, HL 03316 from NIH (D.A.F.), and RR00954 from NIH (Washington University Mass Spectrometry Resource).",

year = "1998",

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doi = "10.1016/S0014-5793(98)00017-9",

language = "English",

volume = "422",

pages = "247--251",

journal = "FEBS Letters",

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T1 - The selective activation of the cardiac sarcolemmal sodium-calcium exchanger by plasmalogenic phosphatidic acid produced by phospholipase D

AU - Hale, Calvin C.

AU - Ebeling, Eric G.

AU - Hsu, Fong F.

AU - Ford, David A.

N1 - Funding Information: This research was supported by the American Heart Association (C.C.H.), HL 42665 from NIH (D.A.F.), Research Career Development Award, HL 03316 from NIH (D.A.F.), and RR00954 from NIH (Washington University Mass Spectrometry Resource).

PY - 1998/1/30

Y1 - 1998/1/30

N2 - Since plasmalogens are the predominant phospholipid of cardiac sarcolemma, the activation of the sodium-calcium exchanger by either plasmenylethanolamine or plasmalogenic phosphatidic acid generated by phospholipase D was explored. Sodium-calcium exchange activity was 7-fold greater in proteoliposomes comprised of plasmenylethanolamine compared to proteoliposomes comprised of only plasmenylcholine. Phospholipase D treatment of proteoliposomes resulted in 1 mol % conversion of plasmenylcholine or phosphatidylcholine to their respective phosphatidis acid molecular species with a concomitant 8-fold or 2-fold activation of sodium-calcium exchange activity, respectfully. Thus, phospholipase D-mediated hydrolysis of plasmalogens to phosphatidic acid may be an important mechanism for the regulation of the sodium-calcium exchanger.

AB - Since plasmalogens are the predominant phospholipid of cardiac sarcolemma, the activation of the sodium-calcium exchanger by either plasmenylethanolamine or plasmalogenic phosphatidic acid generated by phospholipase D was explored. Sodium-calcium exchange activity was 7-fold greater in proteoliposomes comprised of plasmenylethanolamine compared to proteoliposomes comprised of only plasmenylcholine. Phospholipase D treatment of proteoliposomes resulted in 1 mol % conversion of plasmenylcholine or phosphatidylcholine to their respective phosphatidis acid molecular species with a concomitant 8-fold or 2-fold activation of sodium-calcium exchange activity, respectfully. Thus, phospholipase D-mediated hydrolysis of plasmalogens to phosphatidic acid may be an important mechanism for the regulation of the sodium-calcium exchanger.

KW - Myocardium

KW - Phosphatidic acid

KW - Phospholipase D

KW - Plasmalogen

KW - Sodium-calcium exchange

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U2 - 10.1016/S0014-5793(98)00017-9

DO - 10.1016/S0014-5793(98)00017-9

M3 - Article

C2 - 9490017

AN - SCOPUS:0032579194

SN - 0014-5793

VL - 422

SP - 247

EP - 251

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

The selective activation of the cardiac sarcolemmal sodium-calcium exchanger by plasmalogenic phosphatidic acid produced by phospholipase D

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Keywords

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