TY - JOUR
T1 - The secretion of parathormone and glycosylated proteins by parathyroid cells in culture
AU - Morrissey, Jeremiah J.
AU - Hamilton, James W.
AU - Cohn, David V.
N1 - Funding Information:
in part by grant AM 18323 6 Digestive Diseases.
PY - 1978/6/29
Y1 - 1978/6/29
N2 - The secretion of radioactive peptides by dispersed porcine parathyroid cells incubated with [3H]- or [14C]amino acids, [3H]glucosamine and [3H]mannose was analyzed. After incubation, the culture medium contained radioactive parathormone, as expected, and two radioactive glycopeptides: SP I and SP II. SP I appears to be identical with parathyroid secretory protein, heretofore not recognized as a glycoprotein. SP II has not been previously identified. SP I, but not SP II or parathormone, was adsorbed by Concanavalin A possibly reflecting a high mannose content of this molecule. Raising the concentration of calcium in the medium suppressed the secretion of radioactive parathormone and SP I in a similar fashion but did not affect the secretion of SP II. Our results suggest that SP I may play a fundamental role in parathyroid synthetic or secretory processes.
AB - The secretion of radioactive peptides by dispersed porcine parathyroid cells incubated with [3H]- or [14C]amino acids, [3H]glucosamine and [3H]mannose was analyzed. After incubation, the culture medium contained radioactive parathormone, as expected, and two radioactive glycopeptides: SP I and SP II. SP I appears to be identical with parathyroid secretory protein, heretofore not recognized as a glycoprotein. SP II has not been previously identified. SP I, but not SP II or parathormone, was adsorbed by Concanavalin A possibly reflecting a high mannose content of this molecule. Raising the concentration of calcium in the medium suppressed the secretion of radioactive parathormone and SP I in a similar fashion but did not affect the secretion of SP II. Our results suggest that SP I may play a fundamental role in parathyroid synthetic or secretory processes.
UR - http://www.scopus.com/inward/record.url?scp=0018192045&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(78)90326-1
DO - 10.1016/0006-291X(78)90326-1
M3 - Article
C2 - 697794
AN - SCOPUS:0018192045
SN - 0006-291X
VL - 82
SP - 1279
EP - 1286
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -