1 Scopus citations

Abstract

Cells are highly sensitive to their mechanical environment. Understanding the role of the stress environment (e.g., compressive vs. tensile) on tissue development will allow us to engineer replacements for tendons and their fibrocartilaginous bony insertions. We formulated exact linear elasticity and a finite element solution that offered insight into the nature of the compressive environment needed for fibrocartilage formation. The solutions led us to hypothesize that only a single compressive principal stress component is needed to induce cells to synthesize fibrocartilage. These solutions were then validated in an in vitro model. Collagen matrices were seeded with mesenchymal stromal cells and loaded cyclically for 7 days. Immuno-fluorescence was used to determine cell shape, cell orientation, collagen II production, and aggrecan production. We hypothesized that compressive stresses promote the production of fibrocartilage while tensile stresses promote the production of aligned collagenous tissue. This hypothesis was supported by our results: cells in the compressive region were "rounder" than cells in the tensile region, cells in the tensile region aligned with the direction of tension, while the cells in the compressive region had a random orientation, and cells embedded in the collagen matrices produced both aggrecan and type II collagen (components of fibrocartilage).

Original languageEnglish
Title of host publicationProceedings of the 2006 SEM Annual Conference and Exposition on Experimental and Applied Mechanics 2006
Pages2101-2105
Number of pages5
StatePublished - 2006
EventSEM Annual Conference and Exposition on Experimental and Applied Mechanics 2006 - Saint Louis, MO, United States
Duration: Jun 4 2006Jun 7 2006

Publication series

NameProceedings of the 2006 SEM Annual Conference and Exposition on Experimental and Applied Mechanics 2006
Volume4

Conference

ConferenceSEM Annual Conference and Exposition on Experimental and Applied Mechanics 2006
Country/TerritoryUnited States
CitySaint Louis, MO
Period06/4/0606/7/06

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