TY - JOUR
T1 - The role of single-stranded DNA and polymerase α in establishing the ATR, Hus1 DNA replication checkpoint
AU - You, Zhongsheng
AU - Kong, Lindsay
AU - Newport, John
PY - 2002/7/26
Y1 - 2002/7/26
N2 - Using a nucleus-free DNA replication system we have investigated the roles of Xenopus ATR (XATR) and Hus1 (Xhus1) as the DNA replication checkpoint sensors. Like XATR, Xhus1 is required for the checkpoint-dependent phosphorylation of Xchk1 and associates with chromatin in an initiation-dependent manner. While removal of replication protein A inhibits chromatin association of both XATR and Xhus1, removal of polymerase α only disrupts chromatin association of Xhus1. In addition, chromatin association of XATR and Xhus1 are independent of each other. Finally, like XATR, Xhus1 associates with chromatin in unperturbed S phase and dissociates from chromatin following completion of DNA replication.
AB - Using a nucleus-free DNA replication system we have investigated the roles of Xenopus ATR (XATR) and Hus1 (Xhus1) as the DNA replication checkpoint sensors. Like XATR, Xhus1 is required for the checkpoint-dependent phosphorylation of Xchk1 and associates with chromatin in an initiation-dependent manner. While removal of replication protein A inhibits chromatin association of both XATR and Xhus1, removal of polymerase α only disrupts chromatin association of Xhus1. In addition, chromatin association of XATR and Xhus1 are independent of each other. Finally, like XATR, Xhus1 associates with chromatin in unperturbed S phase and dissociates from chromatin following completion of DNA replication.
UR - http://www.scopus.com/inward/record.url?scp=0037178814&partnerID=8YFLogxK
U2 - 10.1074/jbc.M204120200
DO - 10.1074/jbc.M204120200
M3 - Article
C2 - 12015327
AN - SCOPUS:0037178814
SN - 0021-9258
VL - 277
SP - 27088
EP - 27093
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 30
ER -