The role of K+ in the regulation of the increase in intracellular Ca2+ mediated by the T lymphocyte antigen receptor

Lloyd S. Gray, James R. Gnarra, John H. Russell, Victor H. Engelhard

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

The regulation of the increase in intracellular calcium ([Ca2+]i) occurring in cytolytic. T lymphocytes (CTLs) upon their interaction with antigen was examined. This [Ca2+]i increase and lytic function were insensitive to verapamil, a Ca channel blocker. An antigen-independent increase in [Ca2+]i was not induced by depolarization of CTLs with excess extracellular K+, suggesting that Ca2+ influx is not mediated by the ubiquitous voltage-gated Ca channel. The antigen-induced [Ca2+]i increase was inhibited by prior membrane hyperpolarization with valinomycin. Hyperpolarization occurred under normal circumstances in CTLs exposed to antigen-receptor-specific antibodies. This potential change was Ca2+-dependent and inhibited by K channel blockade. Conversely, K channel blockade augmented the antigen-specific [Ca2+]i increase while markedly decreasing the K+ efflux associated with CTL lytic function. Therefore, either membrane potential or intracellular K+ regulates the antigen-specific [Ca2+]i increase in CTLs.

Original languageEnglish
Pages (from-to)119-127
Number of pages9
JournalCell
Volume50
Issue number1
DOIs
StatePublished - Jul 3 1987

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