TY - JOUR
T1 - The role of cell cycle progression for the apoptosis of cancer cells induced by palladium(II)-saccharinate complexes of terpyridine
AU - Kacar, Omer
AU - Cevatemre, Buse
AU - Hatipoglu, Ibrahim
AU - Arda, Nazli
AU - Ulukaya, Engin
AU - Yilmaz, Veysel T.
AU - Acilan, Ceyda
N1 - Publisher Copyright:
© 2017 Elsevier Ltd
PY - 2017
Y1 - 2017
N2 - Objectives Palladium complexes are potent and less toxic molecules in comparison to other metal based agents. Here, we characterized two palladium(II) saccharinate complexes with terpyridine for their cell cycle specificity. Materials and methods Cells were arrested at G1, G1/S boundary or mitosis using mimosine, double-Thymidine block, aphidicolin, nocodazole or colcemid, and evaluated based on morphology and flow cytometry. Synchronized cells were treated with the Pd(II) complexes, and viability was measured via MTT assay. Results While treatment of arrested cells with the Pd(II) complexes resulted in no significant change in cell death in HCT-116 and MDA-MB-231 cells, HeLa cells were more sensitive in S/G1. The main form of cell death was found to be apoptosis. Conclusions Pd(II) complexes appear to be cell-cycle non-specific, while cell line dependent differences may be observed. Cells die through apoptosis regardless of the cell cycle stage, which makes these complexes more promising as anti-cancer agents.
AB - Objectives Palladium complexes are potent and less toxic molecules in comparison to other metal based agents. Here, we characterized two palladium(II) saccharinate complexes with terpyridine for their cell cycle specificity. Materials and methods Cells were arrested at G1, G1/S boundary or mitosis using mimosine, double-Thymidine block, aphidicolin, nocodazole or colcemid, and evaluated based on morphology and flow cytometry. Synchronized cells were treated with the Pd(II) complexes, and viability was measured via MTT assay. Results While treatment of arrested cells with the Pd(II) complexes resulted in no significant change in cell death in HCT-116 and MDA-MB-231 cells, HeLa cells were more sensitive in S/G1. The main form of cell death was found to be apoptosis. Conclusions Pd(II) complexes appear to be cell-cycle non-specific, while cell line dependent differences may be observed. Cells die through apoptosis regardless of the cell cycle stage, which makes these complexes more promising as anti-cancer agents.
KW - Apoptosis
KW - Cancer
KW - Cell cycle arrest
KW - Cell cycle specific
KW - Cytotoxicity
KW - Metal based anticancer agents
KW - Palladium(II) complex
UR - http://www.scopus.com/inward/record.url?scp=85011964410&partnerID=8YFLogxK
U2 - 10.1016/j.bmc.2017.01.033
DO - 10.1016/j.bmc.2017.01.033
M3 - Article
C2 - 28196706
AN - SCOPUS:85011964410
SN - 0968-0896
VL - 25
SP - 1770
EP - 1777
JO - Bioorganic and Medicinal Chemistry
JF - Bioorganic and Medicinal Chemistry
IS - 6
ER -