The rat elastase I regulatory element is an enhancer that directs correct cell specificity and developmental onset of expression in transgenic mice.

R. E. Hammer, G. H. Swift, D. M. Ornitz, C. J. Quaife, R. D. Palmiter, R. L. Brinster, R. J. MacDonald

Research output: Contribution to journalArticle

52 Scopus citations

Abstract

A total of 134 base pairs of the 5' flanking sequence of the elastase I gene is sufficient and necessary to direct expression of the passive human growth hormone gene (hGH) to the exocrine pancreas. We demonstrate that this elastase I regulatory region contains a transcriptional enhancer which directs acinar cell-specific expression in transgenic animals. The elastase I enhancer specifies correct expression of the linked hGH gene in an orientation- and position-independent manner and can activate a heterologous promoter. The enhancer also directs the appropriate temporal activation of the hGH gene in the developing pancreas. Transcription is initiated correctly for the elastase I or hGH promoter, and the transcripts are correctly processed regardless of the enhancer position within or outside the fusion gene. The elastase I enhancer generates coincident DNase I-hypersensitive sites in pancreatic chromatin when moved 3 kilobases upstream or within the first intron of the hGH gene and when associated with the hGH promoter.

Original languageEnglish
Pages (from-to)2956-2967
Number of pages12
JournalMolecular and cellular biology
Volume7
Issue number8
DOIs
StatePublished - Aug 1987

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