TY - JOUR
T1 - The quantification of blood-brain barrier disruption using dynamic contrast-enhanced magnetic resonance imaging in aging rhesus monkeys with spontaneous type 2 diabetes mellitus
AU - Xu, Ziqian
AU - Zeng, Wen
AU - Sun, Jiayu
AU - Chen, Wei
AU - Zhang, Ruzhi
AU - Yang, Zunyuan
AU - Yao, Zunwei
AU - Wang, Lei
AU - Song, Li
AU - Chen, Yushu
AU - Zhang, Yu
AU - Wang, Chunhua
AU - Gong, Li
AU - Wu, Bing
AU - Wang, Tinghua
AU - Zheng, Jie
AU - Gao, Fabao
N1 - Funding Information:
This study was supported by grants 81130027 and 81520108014 from the National Natural Science Foundation of China , grant 2014CB541602 from the National Basic Research Program of China (973 Program) , and grant 2016YFA0201400 from the National Key Research and Development Program of China . The authors also thank Sichuan Primed Bio-Tech Group Co., Ltd., of Chengdu, China, for providing both the diabetic and control monkeys.
Publisher Copyright:
© 2016 Elsevier Inc.
PY - 2017/9
Y1 - 2017/9
N2 - Microvascular lesions of the body are one of the most serious complications that can affect patients with type 2 diabetes mellitus. The blood-brain barrier (BBB) is a highly selective permeable barrier around the microvessels of the brain. This study investigated BBB disruption in diabetic rhesus monkeys using dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI). Multi-slice DCE-MRI was used to quantify BBB permeability. Five diabetic monkeys and six control monkeys underwent magnetic resonance brain imaging in 3 Tesla MRI system. Regions of the frontal cortex, the temporal cortex, the basal ganglia, the thalamus, and the hippocampus in the two groups were selected as regions of interest to calculate the value of the transport coefficient Ktrans using the extended Tofts model. Permeability in the diabetic monkeys was significantly increased as compared with permeability in the normal control monkeys. Histopathologically, zonula occludens protein-1 decreased, immunoglobulin G leaked out of the blood, and nuclear factor E2–related factor translocated from the cytoplasm to the nuclei. It is likely that diabetes contributed to the increased BBB permeability.
AB - Microvascular lesions of the body are one of the most serious complications that can affect patients with type 2 diabetes mellitus. The blood-brain barrier (BBB) is a highly selective permeable barrier around the microvessels of the brain. This study investigated BBB disruption in diabetic rhesus monkeys using dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI). Multi-slice DCE-MRI was used to quantify BBB permeability. Five diabetic monkeys and six control monkeys underwent magnetic resonance brain imaging in 3 Tesla MRI system. Regions of the frontal cortex, the temporal cortex, the basal ganglia, the thalamus, and the hippocampus in the two groups were selected as regions of interest to calculate the value of the transport coefficient Ktrans using the extended Tofts model. Permeability in the diabetic monkeys was significantly increased as compared with permeability in the normal control monkeys. Histopathologically, zonula occludens protein-1 decreased, immunoglobulin G leaked out of the blood, and nuclear factor E2–related factor translocated from the cytoplasm to the nuclei. It is likely that diabetes contributed to the increased BBB permeability.
KW - Blood-brain barrier (BBB)
KW - Dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI)
KW - Extended Tofts model
KW - K
KW - Type 2 diabetes mellitus (T2DM)
UR - http://www.scopus.com/inward/record.url?scp=85019208738&partnerID=8YFLogxK
U2 - 10.1016/j.neuroimage.2016.07.017
DO - 10.1016/j.neuroimage.2016.07.017
M3 - Article
C2 - 27402601
AN - SCOPUS:85019208738
SN - 1053-8119
VL - 158
SP - 480
EP - 487
JO - NeuroImage
JF - NeuroImage
ER -