TY - JOUR
T1 - The Pseudomonas aeruginosa lectin LecB binds to the exopolysaccharide Psl and stabilizes the biofilm matrix
AU - Passos da Silva, Daniel
AU - Matwichuk, Michael L.
AU - Townsend, Delaney O.
AU - Reichhardt, Courtney
AU - Lamba, Doriano
AU - Wozniak, Daniel J.
AU - Parsek, Matthew R.
N1 - Funding Information:
We would like to thank the Analytical Biopharmacy Core, the School of Pharmacy, the Molecular Engineering and Sciences Institute at the University of Washington, and John Sumida for help with ITC experiments. This work was supported by the NIH (R01AI34895, R01AI135309, R01AI077628, and R01AI097511; M.R.P. and D.J.W.).
Publisher Copyright:
© 2019, The Author(s).
PY - 2019/12/1
Y1 - 2019/12/1
N2 - Pseudomonas aeruginosa biofilms are composed of exopolysaccharides (EPS), exogenous DNA, and proteins that hold these communities together. P. aeruginosa produces lectins LecA and LecB, which possess affinities towards sugars found in matrix EPS and mediate adherence of P. aeruginosa to target host cells. Here, we demonstrate that LecB binds to Psl, a key matrix EPS, and this leads to increased retention of both cells and EPS in a growing biofilm. This interaction is predicted to occur between the lectin and the branched side chains present on Psl. Finally, we show that LecB coordinates Psl localization in the biofilm. This constitutes a unique function for LecB and identifies it as a matrix protein that contributes to biofilm structure through EPS interactions.
AB - Pseudomonas aeruginosa biofilms are composed of exopolysaccharides (EPS), exogenous DNA, and proteins that hold these communities together. P. aeruginosa produces lectins LecA and LecB, which possess affinities towards sugars found in matrix EPS and mediate adherence of P. aeruginosa to target host cells. Here, we demonstrate that LecB binds to Psl, a key matrix EPS, and this leads to increased retention of both cells and EPS in a growing biofilm. This interaction is predicted to occur between the lectin and the branched side chains present on Psl. Finally, we show that LecB coordinates Psl localization in the biofilm. This constitutes a unique function for LecB and identifies it as a matrix protein that contributes to biofilm structure through EPS interactions.
UR - http://www.scopus.com/inward/record.url?scp=85065882538&partnerID=8YFLogxK
U2 - 10.1038/s41467-019-10201-4
DO - 10.1038/s41467-019-10201-4
M3 - Article
C2 - 31097723
AN - SCOPUS:85065882538
SN - 2041-1723
VL - 10
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 2183
ER -