TY - JOUR
T1 - The protein network of HIV budding
AU - Von Schwedler, Uta K.
AU - Stuchell, Melissa
AU - Müller, Barbara
AU - Ward, Diane M.
AU - Chung, Hyo Young
AU - Morita, Eiji
AU - Wang, Hubert E.
AU - Davis, Thaylon
AU - He, Gong Ping
AU - Cimbora, Daniel M.
AU - Scott, Anna
AU - Kräusslich, Hans Georg
AU - Kaplan, Jerry
AU - Morham, Scott G.
AU - Sundquist, Wesley I.
N1 - Funding Information:
We thank Cynthia Lodding, Mimi Payne, Collin Kieffer, and Sanaz Ghaffarian for cloning and technical assistance; David Myszka and the University of Utah Protein Interactions facility for Biacore biosensor measurements; Chris Rodesh for confocal microscope training; and Mark Marsh and Heinrich Göttlinger for sharing their unpublished manuscripts. This work was supported by NIH grants to W.I.S. and J.K.
PY - 2003/9/19
Y1 - 2003/9/19
N2 - HIV release requires TSG101, a cellular factor that sorts proteins into vesicles that bud into multivesicular bodies (MVB). To test whether other proteins involved in MVB biogenesis (the class E proteins) also participate in HIV release, we identified 22 candidate human class E proteins. These proteins were connected into a coherent network by 43 different protein-protein interactions, with AIP1 playing a key role in linking complexes that act early (TSG101/ESCRT-I) and late (CHMP4/ESCRT-III) in the pathway. AIP1 also binds the HIV-1 p6Gag and EIAV p9Gag proteins, indicating that it can function directly in virus budding. Human class E proteins were found in HIV-1 particles, and dominant-negative mutants of late-acting human class E proteins arrested HIV-1 budding through plasmal and endosomal membranes. These studies define a protein network required for human MVB biogenesis and indicate that the entire network participates in the release of HIV and probably many other viruses.
AB - HIV release requires TSG101, a cellular factor that sorts proteins into vesicles that bud into multivesicular bodies (MVB). To test whether other proteins involved in MVB biogenesis (the class E proteins) also participate in HIV release, we identified 22 candidate human class E proteins. These proteins were connected into a coherent network by 43 different protein-protein interactions, with AIP1 playing a key role in linking complexes that act early (TSG101/ESCRT-I) and late (CHMP4/ESCRT-III) in the pathway. AIP1 also binds the HIV-1 p6Gag and EIAV p9Gag proteins, indicating that it can function directly in virus budding. Human class E proteins were found in HIV-1 particles, and dominant-negative mutants of late-acting human class E proteins arrested HIV-1 budding through plasmal and endosomal membranes. These studies define a protein network required for human MVB biogenesis and indicate that the entire network participates in the release of HIV and probably many other viruses.
UR - http://www.scopus.com/inward/record.url?scp=10744233294&partnerID=8YFLogxK
U2 - 10.1016/S0092-8674(03)00714-1
DO - 10.1016/S0092-8674(03)00714-1
M3 - Article
C2 - 14505570
AN - SCOPUS:10744233294
SN - 0092-8674
VL - 114
SP - 701
EP - 713
JO - Cell
JF - Cell
IS - 6
ER -