The presence of two local myocardial sheet populations confirmed by diffusion tensor MRI and histological validation

Geoffrey L. Kung, Tom C. Nguyen, Aki Itoh, Stefan Skare, Neil B. Ingels, D. Craig Miller, Daniel B. Ennis

Research output: Contribution to journalArticlepeer-review

58 Scopus citations

Abstract

Purpose: To establish the correspondence between the two histologically observable and diffusion tensor MRI (DTMRI) measurements of myolaminae orientation for the first time and show that single myolaminar orientations observed in local histology may result from histological artifact. Materials and Methods: DTMRI was performed on six sheep left ventricles (LV), then corresponding direct histological transmural measurements were made within the anterobasal and lateral-equatorial LV. Secondary and tertiary eigenvectors of the diffusion tensor were compared with each of the two locally observable sheet orientations from histology. Diffusion tensor invariants were calculated to compare differences in microstructural diffusive properties between histological locations with one observable sheet population and two observable sheet populations. Results: Mean difference ± 1SD between DTMRI and histology measured sheet angles was 8° ± 27°. Diffusion tensor invariants showed no significant differences between histological locations with one observable sheet population and locations with two observable sheet populations. Conclusion: DTMRI measurements of myolaminae orientations derived from the secondary and tertiary eigenvectors correspond to each of the two local myolaminae orientations observed in histology. Two local sheet populations may exist throughout LV myocardium, and one local sheet population observed in histology may be a result of preparation artifact.

Original languageEnglish
Pages (from-to)1080-1091
Number of pages12
JournalJournal of Magnetic Resonance Imaging
Volume34
Issue number5
DOIs
StatePublished - Nov 2011

Keywords

  • DTMRI
  • cardiac sheets
  • histological validation
  • myocardium
  • myofiber

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