TY - JOUR
T1 - The plant-specific histone residue Phe41 is important for genome-wide H3.1 distribution
AU - Lu, Li
AU - Chen, Xiangsong
AU - Qian, Shuiming
AU - Zhong, Xuehua
N1 - Funding Information:
We thank the University of Wisconsin Biotechnology Center DNA Sequencing Facility for providing next-generation sequencing facilities and services. We also thank Dr. Ziqiang Liu for helping with the immunofluorescence work. We are grateful to all members of X. Zhong laboratory for discussions and critical comments on this manuscript. This project was supported by the Alexander von Humboldt Foundation (Alfred Toepfer Faculty Fellow), USDA & National Institute of Food and Agriculture grant (Hatch 1002874), NSF CAREER award (MCB-1552455), and NIH-MIRA grant (R35GM124806).
Publisher Copyright:
© 2018 The Author(s).
PY - 2018/12/1
Y1 - 2018/12/1
N2 - The dynamic incorporation of histone variants influences chromatin structure and many biological processes. In Arabidopsis, the canonical variant H3.1 differs from H3.3 in four residues, one of which (H3.1Phe41) is unique and conserved in plants. However, its evolutionary significance remains unclear. Here, we show that Phe41 first appeared in H3.1 in ferns and became stable during land plant evolution. Unlike H3.1, which is specifically enriched in silent regions, H3.1F41Y variants gain ectopic accumulation at actively transcribed regions. Reciprocal tail and core domain swap experiments between H3.1 and H3.3 show that the H3.1 core, while necessary, is insufficient to restrict H3.1 to silent regions. We conclude that the vascular-plant-specific Phe41 is critical for H3.1 genomic distribution and may act collaboratively with the H3.1 core to regulate deposition patterns. This study reveals that Phe41 may have evolved to provide additional regulation of histone deposition in plants.
AB - The dynamic incorporation of histone variants influences chromatin structure and many biological processes. In Arabidopsis, the canonical variant H3.1 differs from H3.3 in four residues, one of which (H3.1Phe41) is unique and conserved in plants. However, its evolutionary significance remains unclear. Here, we show that Phe41 first appeared in H3.1 in ferns and became stable during land plant evolution. Unlike H3.1, which is specifically enriched in silent regions, H3.1F41Y variants gain ectopic accumulation at actively transcribed regions. Reciprocal tail and core domain swap experiments between H3.1 and H3.3 show that the H3.1 core, while necessary, is insufficient to restrict H3.1 to silent regions. We conclude that the vascular-plant-specific Phe41 is critical for H3.1 genomic distribution and may act collaboratively with the H3.1 core to regulate deposition patterns. This study reveals that Phe41 may have evolved to provide additional regulation of histone deposition in plants.
UR - http://www.scopus.com/inward/record.url?scp=85042036660&partnerID=8YFLogxK
U2 - 10.1038/s41467-018-02976-9
DO - 10.1038/s41467-018-02976-9
M3 - Article
C2 - 29434220
AN - SCOPUS:85042036660
SN - 2041-1723
VL - 9
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 630
ER -